UPSC MainsBOTANY-PAPER-I201110 Marks
Q20.

Describe the following: (c) Stomatal apparatus in Brassica, Dianthus and Poa.

How to Approach

This question requires a comparative account of the stomatal apparatus in three different plant families – Brassicaceae (Brassica), Caryophyllaceae (Dianthus), and Poaceae (Poa). The answer should focus on the structural differences in guard cells, subsidiary cells, and the overall arrangement of the stomatal complex. A clear, concise description of each, followed by a comparative table, will be effective. Diagrams, while not explicitly requested, would enhance the answer significantly in an exam setting.

Model Answer

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Introduction

Stomata are microscopic pores present on the epidermis of leaves and stems, crucial for gas exchange – facilitating the intake of carbon dioxide for photosynthesis and the release of oxygen and water vapor during transpiration. The stomatal apparatus comprises the stomatal pore and the surrounding guard cells, along with subsidiary or accessory cells. The structure of the stomatal apparatus varies considerably across different plant species, reflecting adaptations to diverse environmental conditions. Understanding these variations is fundamental to comprehending plant physiological responses. This answer will detail the stomatal apparatus in *Brassica* (mustard family), *Dianthus* (carnation family), and *Poa* (grass family), highlighting their distinct characteristics.

Stomatal Apparatus in *Brassica*

In *Brassica* (Brassicaceae), the stomata are typically anisocytic, meaning they are surrounded by three subsidiary cells of unequal size. The guard cells are kidney-shaped and possess chloroplasts, enabling them to carry out photosynthesis. The three subsidiary cells are distinct; one is much larger than the other two, and they differ in shape and size. This arrangement provides flexibility and responsiveness to environmental cues, regulating stomatal aperture effectively. The stomata are generally present on both the adaxial (upper) and abaxial (lower) surfaces of the leaves, though more abundant on the abaxial side.

Stomatal Apparatus in *Dianthus*

*Dianthus* (Caryophyllaceae) exhibits a unique type of stomatal apparatus known as diacytic. In this arrangement, each stoma is surrounded by two subsidiary cells, which are wedge-shaped and fit tightly around the guard cells. These subsidiary cells originate from the same mother cell as the guard cells, resulting in a distinct, almost interlocking structure. The guard cells in *Dianthus* are also kidney-shaped and contain chloroplasts. This diacytic type is considered more primitive than anisocytic and is often found in dicotyledonous plants.

Stomatal Apparatus in *Poa*

*Poa* (Poaceae or Gramineae – grass family) displays a parallel-celled or graminaceous type of stomatal apparatus. Here, the guard cells are dumbbell-shaped (elongated with constricted middle) and are flanked by two parallel subsidiary cells on either side. These subsidiary cells are of similar size and shape and run parallel to the long axis of the guard cells. Unlike *Brassica* and *Dianthus*, the subsidiary cells in *Poa* do not differ significantly in size or shape. The stomata in grasses are predominantly found on the lower epidermis of the leaves, an adaptation to minimize water loss in their typically exposed habitats. The long, narrow shape of the guard cells contributes to efficient regulation of the stomatal pore.

Comparative Table of Stomatal Apparatus

Feature *Brassica* (Anisocytic) *Dianthus* (Diacytic) *Poa* (Graminaceous)
Subsidiary Cell Number Three (unequal) Two Two (parallel)
Subsidiary Cell Shape Variable, one large Wedge-shaped Rectangular, parallel
Guard Cell Shape Kidney-shaped Kidney-shaped Dumbbell-shaped
Occurrence Both surfaces, more on abaxial Both surfaces Primarily abaxial
Origin of Subsidiary Cells Different mother cells Same mother cell as guard cells Different mother cells

Conclusion

In conclusion, the stomatal apparatus exhibits significant structural diversity across plant families. *Brassica* demonstrates anisocytic stomata with three unequal subsidiary cells, *Dianthus* displays the diacytic type with two tightly fitting subsidiary cells, and *Poa* features the graminaceous type with two parallel subsidiary cells and dumbbell-shaped guard cells. These variations reflect adaptations to different environmental conditions and contribute to the plant’s ability to regulate gas exchange and water loss effectively. Further research into the genetic and developmental control of stomatal patterning continues to reveal the complexity of this vital plant structure.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Transpiration
The process by which moisture is carried through a plant from the roots to the small pores on the underside of leaves, where it changes to vapor and is released to the atmosphere.
Turgor Pressure
The pressure exerted by the fluid inside a cell against its cell wall. Changes in turgor pressure in guard cells are crucial for regulating stomatal aperture.

Key Statistics

Approximately 90% of water absorbed by plants is lost through transpiration.

Source: Raven, P. H., Evert, R. F., & Eichhorn, S. E. (2013). *Biology of Plants* (8th ed.). W. H. Freeman and Company.

Stomatal conductance, a measure of the rate of CO2 uptake, can vary by a factor of 10 or more within a single day, depending on environmental conditions.

Source: Lambers, H., Chapin, F. S., & Ponsard, S. (2008). *Plant Physiological Ecology*. Springer.

Examples

Xerophytes and Stomatal Adaptations

Plants adapted to arid environments (xerophytes), like cacti, often have sunken stomata and a thick cuticle to reduce water loss through transpiration. This is a clear example of how stomatal structure is linked to environmental adaptation.

Frequently Asked Questions

What role do chloroplasts play in guard cells?

Chloroplasts in guard cells enable them to produce ATP, which is used to actively transport ions (potassium, chloride) into and out of the guard cells. This ion movement changes the turgor pressure, causing the guard cells to swell or shrink, thereby opening or closing the stomatal pore.

Topics Covered

BotanyPlant PhysiologyPlant AnatomyTranspirationGas Exchange