UPSC MainsBOTANY-PAPER-II201115 Marks
Q28.

What are the major functions of cytoskeleton in living cells?

How to Approach

This question requires a detailed understanding of the cytoskeleton's components and their diverse roles within a cell. The answer should be structured around the three main components – microtubules, actin filaments, and intermediate filaments – detailing their structure, protein composition, and specific functions. Emphasis should be placed on how these components contribute to cell shape, movement, intracellular transport, and cell division. A clear and concise explanation of each function, supported by examples, is crucial for a high-scoring answer.

Model Answer

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Introduction

The cytoskeleton is a complex, dynamic network of protein filaments found throughout the cytoplasm of eukaryotic cells. It’s not a static structure, but rather a highly organized and adaptable framework essential for maintaining cell shape, enabling cellular motion, and facilitating intracellular transport. Discovered in the late 19th century, its significance became increasingly apparent with advancements in microscopy and cell biology. The cytoskeleton provides structural support, organizes cellular components, and plays a critical role in fundamental processes like cell division, growth, and signaling. Understanding its functions is paramount to comprehending cellular life.

Major Functions of the Cytoskeleton

The cytoskeleton is composed of three main types of filaments: microtubules, actin filaments (also known as microfilaments), and intermediate filaments. Each type has a unique structure and contributes to different cellular functions.

1. Microtubules

Microtubules are hollow tubes composed of α- and β-tubulin dimers. They are the largest of the three components and are involved in several crucial functions:

  • Cell Shape and Support: Microtubules provide structural support and maintain cell shape, particularly in cells with defined structures like neurons.
  • Intracellular Transport: They serve as tracks for motor proteins (kinesin and dynein) that transport vesicles, organelles, and other cellular cargo throughout the cell.
  • Cell Division: Microtubules form the mitotic spindle, which separates chromosomes during cell division (mitosis and meiosis).
  • Cilia and Flagella: Microtubules are the primary structural component of cilia and flagella, responsible for cell motility and fluid movement.

2. Actin Filaments (Microfilaments)

Actin filaments are thin, flexible strands composed of the protein actin. They are involved in:

  • Cell Motility: Actin filaments interact with myosin motor proteins to generate the force required for cell movement, muscle contraction, and changes in cell shape.
  • Cell Shape and Adhesion: They contribute to cell shape and are involved in forming cell-cell junctions and anchoring cells to the extracellular matrix.
  • Cytokinesis: Actin filaments form a contractile ring that divides the cytoplasm during cell division.
  • Microvilli: Actin filaments are the main component of microvilli, finger-like projections that increase the surface area of cells for absorption.

3. Intermediate Filaments

Intermediate filaments are rope-like structures that provide mechanical strength and stability to cells. They are more permanent structures than microtubules and actin filaments and are involved in:

  • Mechanical Strength: Intermediate filaments resist tensile forces and provide structural support to cells and tissues.
  • Nuclear Lamina: Lamins, a type of intermediate filament, form the nuclear lamina, which supports the nuclear envelope and regulates gene expression.
  • Cell-Cell Junctions: They contribute to the formation of desmosomes and hemidesmosomes, which connect cells to each other and to the extracellular matrix.

The interplay between these three components is crucial for proper cellular function. For example, during cell migration, actin filaments drive the formation of lamellipodia and filopodia, while microtubules provide directional guidance and intermediate filaments provide structural support.

Component Protein Subunit Diameter (nm) Primary Functions
Microtubules α- and β-tubulin 25 Intracellular transport, cell division, cell shape, cilia/flagella
Actin Filaments Actin 7 Cell motility, cell shape, cytokinesis, microvilli
Intermediate Filaments Various (e.g., keratin, vimentin, lamin) 10 Mechanical strength, nuclear support, cell-cell junctions

Conclusion

In conclusion, the cytoskeleton is a remarkably versatile and dynamic network essential for a wide range of cellular processes. Its three main components – microtubules, actin filaments, and intermediate filaments – each contribute unique functions, working in concert to maintain cell shape, enable movement, facilitate intracellular transport, and ensure proper cell division. Disruptions in the cytoskeleton are often associated with various diseases, highlighting its critical role in maintaining cellular health and organismal function. Further research into the intricacies of the cytoskeleton continues to reveal its importance in understanding fundamental biological processes.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Motor Proteins
Proteins that convert chemical energy into mechanical work, enabling movement along the cytoskeleton. Examples include kinesin, dynein, and myosin.
Lamellipodia
Broad, flat, dynamic extensions of the cell membrane driven by actin polymerization, essential for cell migration and exploration of the environment.

Key Statistics

Approximately 40% of a eukaryotic cell's protein mass is dedicated to the cytoskeleton and associated proteins.

Source: Alberts et al., Molecular Biology of the Cell, 6th edition (2015)

Mutations in tubulin genes are associated with a wide range of neurological disorders, affecting approximately 1 in 4,000 births.

Source: Keunen et al., Human Mutation, 2012

Examples

Neutrophil Extracellular Traps (NETs)

Neutrophils, a type of white blood cell, utilize actin filaments to form NETs, web-like structures that trap and kill bacteria. This process is crucial for immune defense.

Frequently Asked Questions

What happens if the cytoskeleton is damaged?

Damage to the cytoskeleton can lead to a variety of cellular dysfunctions, including impaired cell movement, altered cell shape, defects in cell division, and ultimately, cell death. It is implicated in diseases like cancer, neurodegenerative disorders, and muscular dystrophies.

Topics Covered

Cell BiologyCell StructureCell MovementIntracellular Transport