UPSC MainsBOTANY-PAPER-II201220 Marks
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Q6.

What do you mean by aneuploidy and what kinds of chromosomal variations are found in aneuploid plants?

How to Approach

This question requires a detailed understanding of aneuploidy, its causes, and the various chromosomal variations observed in plants. The answer should begin with a clear definition of aneuploidy, differentiating it from euploidy. Subsequently, it should elaborate on different types of aneuploidy (monosomy, trisomy, nullisomy, etc.) and provide examples of aneuploid plants, highlighting the phenotypic effects. A structured approach, utilizing headings and subheadings, will enhance clarity and comprehensiveness.

Model Answer

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Introduction

Aneuploidy, derived from the Greek words ‘aneos’ meaning unequal and ‘ploidy’ meaning number, refers to a condition where the number of chromosomes in a cell deviates from the normal, complete set. Unlike euploidy, which involves changes in the entire genome (whole sets of chromosomes), aneuploidy involves the gain or loss of individual chromosomes. This often arises due to non-disjunction during meiosis, leading to gametes with an abnormal chromosome number. Aneuploidy is a significant cause of genetic disorders in both plants and animals, often resulting in developmental abnormalities and reduced fertility. Understanding the types of aneuploidy and their effects is crucial in plant breeding and evolutionary biology.

Understanding Aneuploidy

Aneuploidy is a type of chromosomal abnormality characterized by the presence of an abnormal number of chromosomes in a cell. This differs from euploidy, where there is a complete set of chromosomes. Aneuploidy can arise due to errors in chromosome segregation during meiosis (non-disjunction) or mitosis. The resulting gametes or somatic cells will have either an extra chromosome or be missing a chromosome.

Types of Aneuploid Variations in Plants

1. Monosomy (2n-1)

Monosomy occurs when one chromosome of a homologous pair is missing. This results in a diploid cell having only one representative of a particular chromosome. Monosomic individuals often exhibit significant phenotypic effects due to gene dosage imbalances. For example, in Datura stramonium, monosomy for chromosome IV leads to reduced pollen viability and stunted growth.

2. Trisomy (2n+1)

Trisomy involves the presence of an extra chromosome, resulting in three copies of a particular chromosome instead of the usual two. Trisomy is generally more tolerable than monosomy, as the extra gene dosage can be partially compensated for. A classic example is trisomy 21 in humans (Down syndrome). In plants, trisomy is frequently induced artificially for breeding purposes. For instance, trisomic strains of wheat (Triticum aestivum) have been used to introduce desirable traits.

3. Nullisomy (2n-2)

Nullisomy refers to the complete absence of a pair of homologous chromosomes. This is usually lethal in diploid organisms due to the severe gene dosage imbalance. However, nullisomic lines can be maintained in some plants through repeated selfing and selection, but they typically exhibit extreme developmental defects.

4. Tetrasomy (2n+2)

Tetrasomy involves the presence of four copies of a particular chromosome. This can occur due to the duplication of a chromosome during meiosis. Tetrasomic plants often show altered phenotypic characteristics, and the extra chromosome copies can contribute to increased genetic diversity.

5. Polysomy (2n+x, where x > 2)

Polysomy refers to the presence of more than two extra chromosomes. This is less common than trisomy but can occur in certain plant species. Polysomic plants often exhibit severe developmental abnormalities and are usually sterile.

Chromosomal Variations and Phenotypic Effects in Aneuploid Plants

Aneuploidy leads to a variety of phenotypic effects in plants, depending on the chromosome involved and the type of aneuploidy. These effects can include:

  • Reduced fertility: Aneuploid plants often exhibit reduced pollen viability and seed set.
  • Stunted growth: The imbalance in gene dosage can disrupt normal growth and development.
  • Morphological abnormalities: Aneuploidy can lead to alterations in leaf shape, flower structure, and other morphological traits.
  • Altered disease resistance: Changes in chromosome number can affect the plant's ability to resist pathogens.
  • Cytological abnormalities: Aneuploid cells often exhibit irregular chromosome pairing during meiosis.

Induced Aneuploidy in Plant Breeding

Aneuploidy can be artificially induced in plants using various techniques, such as:

  • Colchicine treatment: Colchicine is a chemical that inhibits microtubule formation, disrupting chromosome segregation during meiosis and leading to polyploidy and aneuploidy.
  • Temperature shock: Exposure to high or low temperatures can also induce chromosome abnormalities.
  • X-ray irradiation: X-rays can cause chromosome breaks and rearrangements, leading to aneuploidy.

Induced aneuploidy is used in plant breeding to create novel genetic combinations and introduce desirable traits.

Type of Aneuploidy Chromosome Number Phenotypic Effect Example
Monosomy 2n-1 Reduced fertility, stunted growth Datura stramonium (chromosome IV)
Trisomy 2n+1 Altered traits, increased genetic diversity Wheat (Triticum aestivum)
Nullisomy 2n-2 Lethal or severe developmental defects Various plant species (often artificially induced)

Conclusion

Aneuploidy represents a significant form of chromosomal variation in plants, arising from errors in chromosome segregation during cell division. The resulting alterations in chromosome number, manifesting as monosomy, trisomy, nullisomy, and others, lead to a spectrum of phenotypic effects, ranging from reduced fertility to severe developmental abnormalities. While often detrimental, induced aneuploidy serves as a valuable tool in plant breeding for generating genetic diversity and introducing novel traits. Further research into the mechanisms governing aneuploidy and its consequences is crucial for understanding plant evolution and improving crop production.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Non-disjunction
The failure of homologous chromosomes or sister chromatids to separate properly during cell division (meiosis or mitosis), leading to aneuploidy.
Gene Dosage
The number of copies of a gene present in a cell. Aneuploidy alters gene dosage, leading to imbalances in protein production and potentially disrupting cellular function.

Key Statistics

Approximately 5-10% of all human conceptions are estimated to be aneuploid, with the majority resulting in miscarriage. (Source: National Human Genome Research Institute, 2023 - Knowledge Cutoff)

Source: National Human Genome Research Institute

Studies suggest that approximately 1 in 500 live births are affected by aneuploidy, with Down syndrome (trisomy 21) being the most common. (Source: CDC, 2022 - Knowledge Cutoff)

Source: Centers for Disease Control and Prevention (CDC)

Examples

Banana Breeding

Aneuploidy is commonly observed in cultivated bananas (<i>Musa</i> spp.), which are often triploid (2n=3x=54). This triploidy contributes to their seedlessness and fruit size, but also makes them sterile and reliant on vegetative propagation.

Frequently Asked Questions

Is aneuploidy always harmful?

Not always. While often detrimental, aneuploidy can sometimes lead to beneficial traits, particularly in plant breeding. The effects depend on the specific chromosome involved and the type of aneuploidy.

Topics Covered

BiologyPlant ScienceChromosomesGeneticsPlant Breeding