UPSC MainsZOOLOGY-PAPER-II201215 Marks
Q18.

Through a chequerboard, display the various types of genetic code (43). Identify the initiation, termination and nonsense codon, and explain their functions.

How to Approach

This question requires a detailed understanding of the genetic code and its components. The best approach is to first define the genetic code, then present it in a chequerboard format, clearly identifying the initiation, termination, and nonsense codons. Following this, explain the function of each type of codon. The answer should be precise, using biological terminology correctly and demonstrating a clear grasp of the central dogma of molecular biology. Focus on clarity and accuracy in presenting the codon table.

Model Answer

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Introduction

The genetic code is a set of rules used by living cells to translate information encoded within genetic material (DNA or RNA) into proteins. It’s essentially a dictionary that relates codons – three-nucleotide sequences – to specific amino acids. This code is nearly universal across all organisms, demonstrating a common ancestry. Understanding the genetic code is fundamental to comprehending gene expression and the molecular basis of heredity. The question asks for a visual representation of this code, specifically highlighting the crucial roles of initiation, termination, and nonsense codons in the protein synthesis process.

The Genetic Code: A Chequerboard Representation

The genetic code consists of 64 possible codons, formed by combinations of four nucleotide bases: Adenine (A), Guanine (G), Cytosine (C), and Thymine (T) in DNA (or Uracil (U) in RNA). Each codon specifies either an amino acid or a signal to start or stop protein synthesis. The following table represents the standard genetic code, often visualized as a chequerboard:

U C A G
UUU Phe Ser Tyr Cys
UUC Phe Ser Tyr Cys
UUA Leu Pro His Arg
UUG Leu Pro His Arg
UCU Ser Thr Cys Arg
UCC Ser Thr Cys Arg
UCA Ser Thr Leu Arg
UCG Ser Thr Leu Arg
UAU Tyr His Phe Gln
UAC Tyr His Phe Gln
UAA STOP STOP STOP STOP
UAG STOP STOP STOP STOP
UGU Cys Ser Trp Arg
UGC Cys Ser Trp Arg
UGA STOP STOP STOP STOP
UGG Trp

(Note: This table represents only the first page of the complete codon table. The remaining codons follow a similar pattern with A, C, and G in the first position.)

Initiation Codon

The initiation codon is AUG, which codes for the amino acid Methionine (Met). However, in bacteria, a modified form of methionine, formylmethionine (fMet), is often used as the initiating amino acid. The initiation codon signals the ribosome where to begin translating the mRNA sequence. It is crucial for the accurate start of protein synthesis. The initiator tRNA carrying methionine binds to the small ribosomal subunit and then scans the mRNA for the AUG start codon.

Termination Codons

Termination codons, also known as stop codons, signal the end of protein synthesis. There are three termination codons: UAA, UAG, and UGA. These codons do not code for any amino acid. Instead, they are recognized by release factors, which bind to the ribosome and cause the polypeptide chain to be released. Without termination codons, the ribosome would continue translating the mRNA indefinitely, resulting in a non-functional protein.

Nonsense Codons

Nonsense codons are synonymous with termination codons (UAA, UAG, and UGA). The term "nonsense codon" emphasizes their role in creating a premature stop signal during translation. Mutations that introduce a nonsense codon into an mRNA sequence can lead to truncated, non-functional proteins. These mutations are often referred to as "nonsense mutations."

The degeneracy of the genetic code (multiple codons coding for the same amino acid) provides some buffering against the effects of point mutations, but nonsense mutations are generally more detrimental than missense mutations (which change one amino acid for another).

Conclusion

The genetic code is a remarkably conserved system that underpins all life. The accurate identification and function of initiation, termination, and nonsense codons are essential for the faithful translation of genetic information into functional proteins. Understanding these components is crucial for comprehending the molecular mechanisms of inheritance, gene expression, and the consequences of genetic mutations. Further research continues to refine our understanding of the nuances of the genetic code and its role in biological processes.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Codon
A codon is a sequence of three nucleotides in mRNA that specifies a particular amino acid or a termination signal during protein synthesis.
Degeneracy of the genetic code
The degeneracy of the genetic code refers to the fact that most amino acids are specified by more than one codon. This redundancy provides some protection against the effects of mutations.

Key Statistics

The genetic code is nearly universal, with over 99% similarity across all organisms examined (as of 2023).

Source: National Human Genome Research Institute

Approximately 61 codons code for amino acids, while 3 codons serve as stop signals (termination codons).

Source: Molecular Biology of the Gene (Watson et al., 2014)

Examples

Sickle Cell Anemia

A single nucleotide change in the gene for beta-globin (GAG to GTG) results in a substitution of glutamic acid for valine. This seemingly small change alters the protein's structure and function, leading to sickle-shaped red blood cells and the symptoms of sickle cell anemia.

Frequently Asked Questions

What is the wobble hypothesis?

The wobble hypothesis, proposed by Francis Crick, explains why the third base in a codon is often less critical for determining the amino acid. It suggests that the base-pairing rules are "relaxed" at the third position, allowing a single tRNA to recognize multiple codons.

Topics Covered

BiologyGeneticsGenetic CodeCodonsTranslation