UPSC MainsZOOLOGY-PAPER-II201220 Marks
Q14.

Draw a diagram to show the arrangements of various types of contractile proteins in skeletal muscles, and explain the bioenergetics of contraction.

How to Approach

This question requires a combined diagrammatic and explanatory answer. The approach should begin with a clear, labelled diagram of a sarcomere, highlighting the arrangement of actin, myosin, tropomyosin, and troponin. Following the diagram, a detailed explanation of the bioenergetics of muscle contraction, covering ATP hydrolysis, the sliding filament theory, and the role of creatine phosphate, is crucial. The answer should demonstrate understanding of the biochemical processes powering muscle function. Focus on clarity, precision, and scientific accuracy.

Model Answer

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Introduction

Skeletal muscle contraction is fundamental to movement and is a complex process reliant on the intricate interplay of contractile proteins. These proteins, primarily actin and myosin, are organized into repeating units called sarcomeres, the basic functional units of muscle fibers. The energy required for this contraction is derived from the hydrolysis of adenosine triphosphate (ATP), a process coupled with the sliding of these protein filaments past one another. Understanding the arrangement of these proteins and the bioenergetics driving their interaction is essential to comprehending muscle physiology.

Arrangement of Contractile Proteins in Skeletal Muscles

The arrangement of contractile proteins within a skeletal muscle is highly organized. The fundamental unit is the sarcomere, delineated by Z-lines. Within the sarcomere, thick filaments (myosin) and thin filaments (actin) are arranged in a specific pattern.

Sarcomere Diagram

(Diagram of a sarcomere showing the arrangement of actin, myosin, Z-lines, I-band, A-band, H-zone, and M-line. This image is sourced from Wikimedia Commons and is for illustrative purposes only.)

Key Components:

  • Myosin: Thick filaments composed of myosin molecules, each with a globular head that binds to actin.
  • Actin: Thin filaments composed of actin monomers, along with tropomyosin and troponin.
  • Tropomyosin: A protein that winds around actin filaments, blocking myosin-binding sites in a relaxed muscle.
  • Troponin: A complex of proteins that binds to tropomyosin and calcium ions, shifting tropomyosin to expose myosin-binding sites.
  • Z-lines: Boundaries of the sarcomere, to which actin filaments are anchored.
  • I-band: Region containing only actin filaments.
  • A-band: Region containing both actin and myosin filaments.
  • H-zone: Region within the A-band containing only myosin filaments.
  • M-line: Line in the center of the H-zone, anchoring myosin filaments.

Bioenergetics of Muscle Contraction

Muscle contraction is an energy-demanding process, primarily fueled by ATP hydrolysis. The process can be broken down into several key steps:

1. ATP Hydrolysis and Myosin Activation:

ATP binds to the myosin head, causing it to detach from actin. ATP is then hydrolyzed into ADP and inorganic phosphate (Pi) by myosin ATPase, releasing energy. This energy cocks the myosin head into a high-energy conformation, ready to bind to actin.

2. Cross-Bridge Formation:

When calcium ions (Ca2+) are present (released from the sarcoplasmic reticulum following nerve stimulation), they bind to troponin, causing tropomyosin to shift and expose myosin-binding sites on actin. The energized myosin head then binds to actin, forming a cross-bridge.

3. Power Stroke:

The release of Pi from the myosin head triggers the power stroke, where the myosin head pivots, pulling the actin filament towards the center of the sarcomere. This shortens the sarcomere and generates force.

4. Cross-Bridge Detachment:

Another ATP molecule binds to the myosin head, causing it to detach from actin, completing the cycle. If Ca2+ remains present, the cycle repeats, leading to continued contraction.

Energy Sources for Muscle Contraction:

  • ATP: The immediate energy source for muscle contraction. Muscle cells have limited ATP stores.
  • Creatine Phosphate: A high-energy molecule that can rapidly donate a phosphate group to ADP, regenerating ATP. This provides a quick burst of energy.
  • Glycolysis: The breakdown of glucose to produce ATP. This is a faster but less efficient process than oxidative phosphorylation.
  • Oxidative Phosphorylation: The breakdown of glucose, fatty acids, and amino acids in the mitochondria to produce large amounts of ATP. This is the primary source of ATP during prolonged exercise.
Energy System ATP Production Rate ATP Yield Duration
Creatine Phosphate Very Fast Limited (1 ATP per creatine phosphate) ~10-15 seconds
Glycolysis Fast Moderate (2 ATP per glucose) ~30-60 seconds
Oxidative Phosphorylation Slow High (32 ATP per glucose) Prolonged

Conclusion

In conclusion, skeletal muscle contraction is a meticulously orchestrated process dependent on the precise arrangement of contractile proteins and the efficient utilization of energy from ATP hydrolysis. The sliding filament theory, powered by the cyclical attachment, power stroke, and detachment of myosin heads to actin, explains the mechanism of muscle shortening. Understanding the interplay between these proteins and the various energy systems that replenish ATP is crucial for comprehending muscle function and its implications for overall physiological performance. Further research continues to refine our understanding of muscle physiology, particularly in the context of muscle fatigue and disease.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Sarcomere
The basic functional unit of a muscle fiber, defined as the segment between two successive Z-lines. It is the fundamental unit responsible for muscle contraction.
Myosin ATPase
An enzyme located on the myosin head that hydrolyzes ATP into ADP and inorganic phosphate, providing the energy for the power stroke during muscle contraction.

Key Statistics

Approximately 40-60% of the human body mass is comprised of skeletal muscle.

Source: Guyton and Hall Textbook of Medical Physiology (Knowledge cutoff 2023)

Skeletal muscle accounts for approximately 85% of the total ATP consumption in the human body at rest.

Source: Principles of Anatomy and Physiology, Tortora & Derrickson (Knowledge cutoff 2023)

Examples

Muscle Cramps

Muscle cramps often occur due to electrolyte imbalances (e.g., potassium, calcium, magnesium) which disrupt the normal functioning of muscle contraction and relaxation, leading to sustained, involuntary muscle contractions.

Frequently Asked Questions

What role does calcium play in muscle contraction?

Calcium ions act as a key signaling molecule. They bind to troponin, causing a conformational change that moves tropomyosin and exposes the myosin-binding sites on actin, allowing cross-bridge formation and initiating contraction.

Topics Covered

BiologyPhysiologyMuscle ContractionBioenergeticsSkeletal Muscle