UPSC MainsZOOLOGY-PAPER-II201420 Marks
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Q8.

Compare the events during mitotic and meiotic cell cycles. Add a note on role of stage-specific macromolecules and enzymes in such cycles.

How to Approach

This question requires a comparative analysis of mitosis and meiosis, focusing on the events occurring in each cell cycle stage. The answer should begin by defining both processes and then systematically compare their phases (prophase, metaphase, anaphase, telophase) highlighting key differences. Crucially, the role of stage-specific macromolecules (like cyclins, CDKs) and enzymes (like separase, cohesin) needs to be discussed. A tabular format will be helpful for comparison. The answer should demonstrate a strong understanding of cell biology and genetics.

Model Answer

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Introduction

Cell division is fundamental to life, enabling growth, repair, and reproduction. Two primary types of cell division occur in eukaryotes: mitosis and meiosis. Mitosis results in two genetically identical daughter cells, crucial for growth and asexual reproduction. Meiosis, on the other hand, is a reduction division that produces four genetically diverse haploid cells, essential for sexual reproduction. Both processes involve a series of precisely orchestrated stages, governed by specific macromolecules and enzymes. Understanding the differences and similarities between these cycles is vital for comprehending inheritance, genetic variation, and developmental biology.

Mitosis vs. Meiosis: A Comparative Analysis

Both mitosis and meiosis are continuous processes, but are conventionally divided into phases for ease of understanding: Prophase, Metaphase, Anaphase, and Telophase (M phase). However, significant differences exist in the events occurring within each phase.

Mitosis

Mitosis is a single nuclear division resulting in two identical daughter cells. It’s divided into:

  • Prophase: Chromosomes condense, the nuclear envelope breaks down, and the mitotic spindle begins to form.
  • Metaphase: Chromosomes align at the metaphase plate, attached to spindle fibers from opposite poles.
  • Anaphase: Sister chromatids separate and move towards opposite poles, pulled by the shortening spindle fibers.
  • Telophase: Chromosomes arrive at the poles, the nuclear envelope reforms, and the chromosomes decondense. Cytokinesis (cell division) usually occurs concurrently.

Meiosis

Meiosis consists of two successive nuclear divisions: Meiosis I and Meiosis II.

  • Meiosis I: This is a reduction division, halving the chromosome number.
    • Prophase I: A complex phase with five substages (Leptotene, Zygotene, Pachytene, Diplotene, Diakinesis). Homologous chromosomes pair up (synapsis) forming tetrads, and crossing over occurs, leading to genetic recombination.
    • Metaphase I: Tetrads align at the metaphase plate.
    • Anaphase I: Homologous chromosomes separate, but sister chromatids remain attached.
    • Telophase I: Chromosomes arrive at the poles, and the cell divides, resulting in two haploid cells.
  • Meiosis II: Similar to mitosis, but with haploid cells.
    • Prophase II: Chromosomes condense.
    • Metaphase II: Chromosomes align at the metaphase plate.
    • Anaphase II: Sister chromatids separate.
    • Telophase II: Chromosomes arrive at the poles, and the cells divide, resulting in four haploid daughter cells.

Comparative Table

Feature Mitosis Meiosis I Meiosis II
Number of Divisions 1 1 1
Daughter Cells 2 2 4
Chromosome Number Same as parent (2n → 2n) Reduced by half (2n → n) Remains haploid (n → n)
Genetic Variation None High (crossing over, independent assortment) None
Homologous Chromosome Pairing No Yes (Synapsis) No
Separation of Sister Chromatids Anaphase Anaphase I (Homologous chromosomes separate) Anaphase II (Sister chromatids separate)

Role of Stage-Specific Macromolecules and Enzymes

Cell cycle progression is tightly regulated by a complex interplay of macromolecules and enzymes.

  • Cyclins and Cyclin-Dependent Kinases (CDKs): These are key regulators. Cyclins accumulate during specific phases and bind to CDKs, activating them. Different cyclin-CDK complexes trigger different events (e.g., DNA replication, chromosome condensation).
  • Cohesins: These protein complexes hold sister chromatids together after DNA replication. They are cleaved by separase during anaphase, allowing chromatid separation.
  • Condensins: These proteins help in chromosome condensation during prophase.
  • MPF (Maturation Promoting Factor): A cyclin-CDK complex crucial for initiating mitosis.
  • Anaphase Promoting Complex/Cyclosome (APC/C): An E3 ubiquitin ligase that targets proteins for degradation, including securin (which inhibits separase), leading to anaphase onset.
  • Recombinases: Enzymes involved in crossing over during Prophase I of meiosis.

Dysregulation of these molecules can lead to errors in cell division, potentially causing cancer or genetic disorders.

Conclusion

In conclusion, mitosis and meiosis are distinct cell division processes with differing outcomes and regulatory mechanisms. Mitosis ensures faithful replication for growth and repair, while meiosis generates genetic diversity essential for sexual reproduction. The precise orchestration of these cycles relies on the coordinated action of stage-specific macromolecules and enzymes, highlighting the intricate control mechanisms governing cell division. Understanding these processes is crucial for advancements in fields like genetics, developmental biology, and cancer research.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Synapsis
The pairing of homologous chromosomes during prophase I of meiosis, forming a tetrad, which is essential for crossing over.
Haploid
A cell or organism having only one set of chromosomes (n). In humans, haploid cells are gametes (sperm and egg).

Key Statistics

Approximately 90% of human cancers are associated with genetic instability and defects in cell cycle control mechanisms.

Source: American Cancer Society (Knowledge cutoff: 2023)

The human genome contains approximately 20,000-25,000 genes, and errors in cell division contribute to mutations in these genes.

Source: National Human Genome Research Institute (Knowledge cutoff: 2023)

Examples

Down Syndrome

Down syndrome (Trisomy 21) is a genetic disorder caused by the presence of an extra copy of chromosome 21, often resulting from non-disjunction during meiosis.

Frequently Asked Questions

What is the significance of crossing over in meiosis?

Crossing over generates genetic recombination, increasing genetic diversity among offspring. This diversity is crucial for adaptation and evolution.

Topics Covered

BiologyCell BiologyGeneticsCell CycleMitosisMeiosis