UPSC MainsAGRICULTURE-PAPER-I201512 Marks
Q17.

What are the components of DNA and RNA? Give the characteristic difference of the DNA and RNA.

How to Approach

This question requires a clear understanding of the molecular basis of life. The approach should be to first define DNA and RNA, outlining their basic components (nucleotides, sugar, phosphate group, nitrogenous bases). Then, detail the composition of each, including the specific nitrogenous bases. Finally, systematically compare and contrast DNA and RNA, highlighting key structural and functional differences. A tabular format would be beneficial for highlighting the differences. Emphasis should be placed on the significance of these differences in biological processes.

Model Answer

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Introduction

Deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) are the two essential nucleic acids responsible for carrying and expressing genetic information within living organisms. DNA serves as the blueprint for life, containing instructions for building and maintaining an organism. RNA, on the other hand, plays diverse roles in the processes of protein synthesis and gene regulation. The discovery of the structure of DNA by Watson and Crick in 1953 revolutionized our understanding of heredity. This answer will detail the components of both DNA and RNA, and then contrast their key characteristics, highlighting their distinct roles in cellular processes.

Components of DNA

DNA, or deoxyribonucleic acid, is a polymer composed of repeating units called nucleotides. Each nucleotide consists of three components:

  • Deoxyribose Sugar: A five-carbon sugar.
  • Phosphate Group: A negatively charged group.
  • Nitrogenous Base: There are four types in DNA: Adenine (A), Guanine (G), Cytosine (C), and Thymine (T).

These nucleotides are linked together by phosphodiester bonds, forming a long chain. The sequence of nitrogenous bases encodes the genetic information.

Components of RNA

RNA, or ribonucleic acid, is also a polymer of nucleotides, but with some key differences from DNA.

  • Ribose Sugar: A five-carbon sugar (unlike DNA's deoxyribose).
  • Phosphate Group: Similar to DNA.
  • Nitrogenous Bases: RNA contains Adenine (A), Guanine (G), Cytosine (C), and Uracil (U) instead of Thymine (T). Uracil replaces Thymine.

RNA can exist as a single strand or, in some cases, fold back on itself to form double-stranded structures.

Key Differences Between DNA and RNA

The table below summarizes the key differences between DNA and RNA:

Feature DNA RNA
Sugar Deoxyribose Ribose
Nitrogenous Bases Adenine, Guanine, Cytosine, Thymine Adenine, Guanine, Cytosine, Uracil
Strand Structure Double helix (usually) Single strand (usually)
Stability More stable Less stable
Location Primarily in the nucleus Nucleus and cytoplasm
Function Genetic information storage; replication Protein synthesis; gene regulation

Further Elaboration on Differences

The differences in sugar and nitrogenous bases significantly impact the properties and functions of DNA and RNA.

  • Sugar Difference: The presence of a hydroxyl group (-OH) on the 2' carbon of ribose in RNA makes it more reactive and less stable than DNA. This instability is crucial for RNA’s role as a temporary messenger.
  • Base Difference: The substitution of Thymine (T) with Uracil (U) in RNA alters hydrogen bonding patterns and influences RNA’s structural flexibility. Uracil pairs with Adenine just like Thymine does in DNA.
  • Structure: DNA’s double helix provides stability and protection for the genetic code. RNA's single-stranded nature allows for a greater range of structural conformations, enabling diverse functions.

Types of RNA

RNA exists in various forms, each with a specific role:

  • mRNA (messenger RNA): Carries genetic information from DNA to ribosomes.
  • tRNA (transfer RNA): Transports amino acids to ribosomes during protein synthesis.
  • rRNA (ribosomal RNA): Forms part of the ribosome structure and catalyzes peptide bond formation.
  • miRNA (microRNA): Regulates gene expression by binding to mRNA.
Example 1: CRISPR-Cas9 System: This revolutionary gene-editing technology utilizes RNA molecules (guide RNA) to direct the Cas9 enzyme to specific DNA sequences, enabling precise modifications to the genome. This highlights the functional importance of RNA in manipulating genetic material. Case Study: SARS-CoV-2 RNA Genome: The SARS-CoV-2 virus, responsible for the COVID-19 pandemic, has an RNA genome. Understanding the structure and function of this RNA genome has been crucial for developing vaccines and antiviral therapies. The rapid development of mRNA vaccines, for example, is a testament to the power of RNA-based technologies.

Conclusion

In conclusion, DNA and RNA are fundamental nucleic acids with distinct structures and functions. While both are composed of nucleotides, the differences in their sugar and base composition, as well as their typical structural forms, dictate their unique roles in the central dogma of molecular biology. DNA serves as the stable repository of genetic information, while RNA acts as a versatile intermediary in gene expression and regulation. Ongoing research continues to unveil new and complex roles for RNA, highlighting its significance in biological processes and its potential for therapeutic interventions.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Nucleotide
The basic building block of DNA and RNA, composed of a sugar, phosphate group, and nitrogenous base.
Phosphodiester Bond
The chemical bond that links nucleotides together in a DNA or RNA chain.

Key Statistics

The human genome contains approximately 3 billion base pairs of DNA.

Source: National Human Genome Research Institute

The average gene length in the human genome is about 1500 base pairs.

Source: NHGRI

Examples

RNA Interference (RNAi)

A biological process where small RNA molecules (like siRNA and miRNA) silence gene expression by targeting mRNA molecules for degradation or translational repression. It’s being explored for therapeutic applications.

Frequently Asked Questions

What is the significance of Uracil in RNA?

Uracil replaces Thymine in RNA, still allowing for base pairing with Adenine, but its presence contributes to RNA's overall reactivity and functionality.

Topics Covered

BiologyGeneticsBiochemistryNucleic AcidsDNA StructureRNA Structure