UPSC MainsBOTANY-PAPER-I201610 Marks150 Words
Q2.

Ultrastructure of flagella in Algae

How to Approach

This question requires a detailed understanding of the ultrastructure of algal flagella, going beyond a simple description. The answer should focus on the '9+2' arrangement of microtubules, the associated proteins, the basal body structure, and any variations observed in different algal groups. A comparative approach, highlighting differences between algal flagella and those of other organisms, would be beneficial. Structure should be: Introduction defining flagella and its role, detailed body covering the ultrastructure, and a concise conclusion.

Model Answer

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Introduction

Flagella are filamentous appendages that protrude from the cell surface and are primarily responsible for motility in many algal species. These structures are crucial for navigating their aquatic environments, facilitating nutrient acquisition, and reproduction. The algal flagellum, while functionally similar to those found in other eukaryotic organisms, exhibits a unique ultrastructure that has been extensively studied using electron microscopy. Understanding this ultrastructure is fundamental to comprehending algal locomotion and evolution. This note will detail the intricate components and organization of algal flagella.

Ultrastructure of Algal Flagella

The ultrastructure of algal flagella is remarkably conserved across diverse algal groups, yet subtle variations exist. The core structure is based on the ‘9+2’ arrangement of microtubules, a hallmark of eukaryotic flagella and cilia.

1. Microtubule Arrangement

The ‘9+2’ arrangement refers to nine doublet microtubules arranged in a circle around a central pair of single microtubules. Each doublet consists of one complete microtubule (A-tubule) and one incomplete microtubule (B-tubule). These microtubules are composed of the protein tubulin.

2. Dynein Arms

Attached to each A-tubule of the doublet microtubules are dynein arms. These are large, multi-subunit protein complexes that generate the force for flagellar bending. Dynein arms utilize ATP hydrolysis to ‘walk’ along the adjacent B-tubule, causing the microtubules to slide past each other. This sliding is constrained by other proteins, resulting in bending rather than complete separation.

3. Radial Spokes

Radial spokes extend from each doublet microtubule towards the central pair. These spokes are thought to regulate dynein activity and coordinate the bending motion. They connect to a central sheath surrounding the central pair of microtubules.

4. Nexin Links

Nexin links are elastic protein linkages that connect adjacent doublet microtubules. They resist the sliding force generated by dynein, converting it into bending. Different types of nexin links (inner and outer) contribute to the flagellum’s structural integrity and flexibility.

5. Basal Body

The flagellum originates from a basal body, which is structurally identical to a centriole. The basal body is a cylindrical structure composed of nine triplet microtubules. It serves as the nucleation site for flagellar microtubules and anchors the flagellum to the cell. The transition zone, located at the base of the flagellum, connects the basal body to the flagellar shaft and regulates the entry of proteins into the flagellum.

6. Variations in Algal Flagella

  • Mastigonemes: Some algal groups, like certain chrysophytes, possess mastigonemes – hair-like appendages extending from the flagellar shaft. These are composed of proteins and contribute to increased surface area and hydrodynamic properties.
  • Scales: Certain algae exhibit scales on their flagella, providing additional structural support and potentially influencing water flow.
  • Rootlets: Rootlets are fibrous structures that extend from the basal body into the cytoplasm, providing structural support and anchoring the flagellum.

Comparison with Animal Flagella: While the basic ‘9+2’ structure is conserved, algal flagella often lack the complex outer dynein arms found in many animal flagella. This difference may reflect adaptations to different hydrodynamic environments and motility strategies.

Component Description
Microtubules ‘9+2’ arrangement of doublet and single microtubules.
Dynein Arms Protein complexes responsible for generating force.
Radial Spokes Regulate dynein activity and coordinate bending.
Nexin Links Connect doublet microtubules and resist sliding.
Basal Body Anchors the flagellum and serves as a nucleation site.

Conclusion

In conclusion, the ultrastructure of algal flagella is a highly organized and complex system based on the ‘9+2’ microtubule arrangement. The coordinated action of dynein arms, radial spokes, and nexin links enables efficient and controlled movement. Variations in flagellar appendages, such as mastigonemes and scales, reflect adaptations to specific ecological niches. Further research into the molecular mechanisms governing flagellar function will continue to enhance our understanding of algal motility and evolution.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Dynein
A motor protein responsible for generating force in flagella and cilia through ATP hydrolysis, causing microtubule sliding.
Mastigoneme
A hair-like appendage found on the flagella of some algae, composed of proteins and contributing to increased surface area and hydrodynamic properties.

Key Statistics

Approximately 70% of algal species are motile, relying on flagella for at least part of their life cycle.

Source: Andersen, R. A. (2005). Algal culturing techniques. Elsevier Academic Press.

Electron microscopy reveals that the flagellar membrane contains approximately 60 different proteins.

Source: Gilson, P. R., & Pan, Y. (2013). The flagellar membrane proteome.

Examples

Chlamydomonas

The green alga *Chlamydomonas reinhardtii* is a model organism for studying flagellar structure and function. Its two flagella are essential for swimming and phototaxis.

Frequently Asked Questions

What is the role of the central pair of microtubules?

The central pair of microtubules is thought to play a role in coordinating flagellar bending and maintaining structural integrity, although its exact function is still being investigated.

Topics Covered

BotanyBiologyAlgaeCell structureFlagella