UPSC MainsMEDICAL-SCIENCE-PAPER-I201615 Marks
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Q11.

Outline the biochemical functions and the deficiency symptoms of sulphur-containing vitamins.

How to Approach

This question requires a detailed understanding of sulphur-containing vitamins – Biotin, Thiamine, and Pantothenic Acid – focusing on their biochemical roles and the consequences of their deficiencies. The answer should be structured to cover each vitamin individually, outlining its coenzyme form, key metabolic functions, and specific deficiency symptoms. A comparative approach can be used to highlight similarities and differences. Focus on clinical manifestations and biochemical basis of the symptoms.

Model Answer

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Introduction

Sulphur-containing vitamins are crucial for numerous metabolic processes within the human body. These vitamins, namely Biotin (Vitamin B7), Thiamine (Vitamin B1), and Pantothenic Acid (Vitamin B5), often function as components of coenzymes, assisting enzymes in catalyzing vital biochemical reactions. Their deficiencies, though relatively uncommon due to widespread availability in food, can lead to a range of clinical manifestations affecting various organ systems. Understanding their biochemical functions and associated deficiency symptoms is essential for accurate diagnosis and effective management of nutritional disorders.

Biotin (Vitamin B7)

Biotin serves as a coenzyme for carboxylase enzymes, which are involved in carboxylation reactions crucial for fatty acid synthesis, gluconeogenesis, and amino acid catabolism. The primary coenzyme form is Biotinyl-lysine.

  • Biochemical Functions:
    • Fatty Acid Synthesis: Acetyl-CoA carboxylase utilizes biotin to convert acetyl-CoA to malonyl-CoA, a rate-limiting step in fatty acid synthesis.
    • Gluconeogenesis: Pyruvate carboxylase uses biotin to convert pyruvate to oxaloacetate, an essential step in glucose production from non-carbohydrate sources.
    • Amino Acid Metabolism: Propionyl-CoA carboxylase utilizes biotin in the breakdown of certain amino acids (valine, isoleucine, methionine, and threonine).
  • Deficiency Symptoms:
    • Dermatitis: Scaly, red rash around the eyes, nose, and mouth.
    • Alopecia: Hair loss, often diffuse.
    • Neurological Symptoms: Depression, lethargy, hallucinations, and paresthesia (numbness and tingling).
    • Metabolic Acidosis: Impaired gluconeogenesis can lead to metabolic acidosis.

Thiamine (Vitamin B1)

Thiamine is a vital component of Thiamine Pyrophosphate (TPP), a coenzyme essential for carbohydrate metabolism. It plays a critical role in the decarboxylation of α-keto acids and transketolase reactions.

  • Biochemical Functions:
    • Pyruvate Dehydrogenase Complex: TPP is a coenzyme for pyruvate dehydrogenase, converting pyruvate to acetyl-CoA, linking glycolysis to the Krebs cycle.
    • α-Ketoglutarate Dehydrogenase Complex: TPP is also a coenzyme for α-ketoglutarate dehydrogenase, a key enzyme in the Krebs cycle.
    • Transketolase: TPP is essential for transketolase, an enzyme in the pentose phosphate pathway, crucial for NADPH production and ribose-5-phosphate synthesis.
  • Deficiency Symptoms:
    • Beriberi: Characterized by neurological and cardiovascular symptoms.
      • Dry Beriberi: Primarily affects the nervous system, causing peripheral neuropathy, muscle weakness, and ataxia.
      • Wet Beriberi: Affects the cardiovascular system, leading to heart failure, edema, and shortness of breath.
    • Wernicke-Korsakoff Syndrome: Seen in chronic alcoholics, characterized by confusion, ataxia, ophthalmoplegia (eye paralysis), and memory impairment (Korsakoff psychosis).

Pantothenic Acid (Vitamin B5)

Pantothenic acid is a component of Coenzyme A (CoA), a crucial coenzyme involved in numerous metabolic pathways, including fatty acid metabolism, carbohydrate metabolism, and protein metabolism.

  • Biochemical Functions:
    • Fatty Acid Metabolism: CoA is essential for the synthesis and degradation of fatty acids.
    • Krebs Cycle: CoA carries acetyl groups into the Krebs cycle.
    • Cholesterol Synthesis: CoA is involved in cholesterol biosynthesis.
    • Acylation Reactions: CoA participates in the acylation of proteins, carbohydrates, and other molecules.
  • Deficiency Symptoms:

    Pantothenic acid deficiency is extremely rare due to its widespread presence in foods. However, experimental deficiencies have shown:

    • Burning Feet Syndrome: Characterized by pain, numbness, and burning sensations in the feet.
    • Fatigue: General weakness and tiredness.
    • Headache: Frequent and severe headaches.
    • Sleep Disturbances: Insomnia and other sleep problems.
    • Gastrointestinal Distress: Nausea, vomiting, and abdominal cramps.
Vitamin Coenzyme Form Key Biochemical Function Deficiency Symptoms
Biotin Biotinyl-lysine Carboxylation reactions (fatty acid synthesis, gluconeogenesis) Dermatitis, alopecia, neurological symptoms
Thiamine Thiamine Pyrophosphate (TPP) Carbohydrate metabolism (pyruvate dehydrogenase, transketolase) Beriberi, Wernicke-Korsakoff Syndrome
Pantothenic Acid Coenzyme A (CoA) Fatty acid metabolism, Krebs cycle, cholesterol synthesis Burning feet syndrome, fatigue, headache

Conclusion

Sulphur-containing vitamins are indispensable for maintaining metabolic homeostasis. While overt deficiencies are uncommon, subtle insufficiencies can contribute to a variety of health problems. Understanding the specific biochemical roles of Biotin, Thiamine, and Pantothenic Acid, and recognizing the associated deficiency symptoms, is crucial for healthcare professionals. Further research into the optimal intake levels and potential benefits of these vitamins remains an important area of investigation, particularly in the context of specific populations and dietary patterns.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Coenzyme
A non-protein chemical compound that is bound to an enzyme and is required for the enzyme to carry out its catalytic activity.
Metabolic Acidosis
A condition characterized by a decrease in blood pH due to an accumulation of acid, often resulting from impaired metabolic processes.

Key Statistics

Globally, an estimated 1 billion people suffer from some form of vitamin deficiency (WHO, 2021 - knowledge cutoff).

Source: World Health Organization (WHO)

Approximately 20-30% of individuals with chronic alcohol use disorder develop thiamine deficiency (National Institute on Alcohol Abuse and Alcoholism, 2019 - knowledge cutoff).

Source: National Institute on Alcohol Abuse and Alcoholism (NIAAA)

Examples

Raw Egg White and Biotin Deficiency

Avidin, a protein found in raw egg white, binds strongly to biotin, preventing its absorption. Historically, consuming large amounts of raw egg whites led to biotin deficiency.

Frequently Asked Questions

Can stress affect vitamin B5 levels?

Yes, chronic stress can deplete pantothenic acid levels as it is heavily involved in adrenal hormone production.

Topics Covered

BiochemistryNutritionVitaminsMetabolismNutritional Deficiencies