UPSC MainsBOTANY-PAPER-II201710 Marks
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Q1.

Draw a labelled diagram of prokaryotic and eukaryotic chloroplast and describe their functions.

How to Approach

This question requires a detailed understanding of plant cell biology, specifically focusing on chloroplasts. The approach should involve drawing clear, labelled diagrams of both prokaryotic (cyanobacteria) and eukaryotic (plant cell) chloroplasts, highlighting their structural differences. Following the diagrams, a comprehensive description of the functions of chloroplasts, including photosynthesis and other metabolic roles, is crucial. The answer should demonstrate knowledge of the endosymbiotic theory explaining the origin of chloroplasts.

Model Answer

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Introduction

Chloroplasts are the organelles responsible for photosynthesis in plants and algae. These organelles convert light energy into chemical energy, fueling most life on Earth. While commonly associated with eukaryotic cells, chloroplast-like structures are also found in prokaryotic organisms like cyanobacteria. The evolutionary history of chloroplasts is deeply rooted in the endosymbiotic theory, which posits that these organelles originated from ancient bacteria engulfed by eukaryotic cells. Understanding the structural differences and functional similarities between prokaryotic and eukaryotic chloroplasts is fundamental to comprehending plant biology and the evolution of photosynthesis.

Prokaryotic Chloroplast (Cyanobacteria)

Cyanobacteria, formerly known as blue-green algae, are photosynthetic prokaryotes. Their photosynthetic machinery is not compartmentalized within a defined chloroplast like in eukaryotes, but rather distributed within the cytoplasm, associated with internal membrane systems called thylakoids.

Prokaryotic Chloroplast
  • Structure: Lacks membrane-bound organelles. Photosynthetic pigments (chlorophyll a) and enzymes are located on thylakoid membranes. These thylakoids are often arranged in stacks or peripheral regions.
  • Thylakoids: Not organized into grana stacks as in eukaryotic chloroplasts.
  • DNA: Contains a circular DNA molecule (nucleoid) encoding photosynthetic genes.
  • Ribosomes: Contains 70S ribosomes.

Eukaryotic Chloroplast (Plant Cell)

Eukaryotic chloroplasts are more complex organelles found within plant cells and algae. They are enclosed by a double membrane and contain an intricate internal membrane system.

Eukaryotic Chloroplast
  • Structure: Enclosed by a double membrane – outer and inner membrane. Contains internal membrane system called thylakoids, arranged in stacks called grana.
  • Grana: Stacks of thylakoids where the light-dependent reactions of photosynthesis occur.
  • Stroma: The fluid-filled space surrounding the thylakoids, where the light-independent reactions (Calvin cycle) take place.
  • DNA: Contains its own circular DNA genome, supporting the endosymbiotic theory.
  • Ribosomes: Contains 80S ribosomes.
  • Plastids: Chloroplasts are part of a family of organelles called plastids, which also include leucoplasts (storage) and chromoplasts (pigment storage).

Functions of Chloroplasts

Photosynthesis

The primary function of chloroplasts is photosynthesis, the process of converting light energy into chemical energy in the form of glucose. This process involves two main stages:

  • Light-dependent reactions: Occur in the thylakoid membranes. Light energy is absorbed by chlorophyll and used to split water molecules, releasing oxygen, protons, and electrons. ATP and NADPH are produced.
  • Light-independent reactions (Calvin cycle): Occur in the stroma. Carbon dioxide is fixed and converted into glucose using the ATP and NADPH generated during the light-dependent reactions.

Other Functions

  • Amino acid synthesis: Chloroplasts participate in the synthesis of some amino acids.
  • Fatty acid synthesis: They contribute to the synthesis of fatty acids.
  • Starch synthesis: Glucose produced during photosynthesis is often stored as starch within the chloroplasts.
  • Protein synthesis: Chloroplasts have their own protein synthesis machinery, allowing them to produce many of the proteins required for their function.

Comparison Table

Feature Prokaryotic Chloroplast (Cyanobacteria) Eukaryotic Chloroplast (Plant Cell)
Membrane Structure No defined chloroplast; thylakoids within cytoplasm Double membrane-bound organelle
Thylakoid Arrangement Not organized into grana Organized into grana stacks
DNA Circular DNA (nucleoid) Circular DNA genome
Ribosomes 70S 80S
Complexity Simpler More complex

Conclusion

In conclusion, both prokaryotic and eukaryotic chloroplasts are vital for photosynthesis, but they differ significantly in their structural organization. The prokaryotic version in cyanobacteria represents a simpler, less compartmentalized system, while the eukaryotic chloroplast is a more complex organelle with a double membrane and organized thylakoid stacks. The endosymbiotic theory provides a compelling explanation for the origin of eukaryotic chloroplasts, highlighting the evolutionary relationship between these organelles and ancient bacteria. Understanding these differences is crucial for appreciating the diversity and complexity of photosynthetic life on Earth.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Endosymbiotic Theory
The endosymbiotic theory proposes that eukaryotic organelles, such as chloroplasts and mitochondria, originated as free-living bacteria that were engulfed by ancestral eukaryotic cells and established a symbiotic relationship.
Thylakoid
A flattened sac-like membrane within the chloroplast where the light-dependent reactions of photosynthesis take place. Thylakoids contain chlorophyll and other photosynthetic pigments.

Key Statistics

Photosynthesis is estimated to convert over 100 billion metric tons of carbon dioxide into organic matter annually.

Source: NASA Earth Observatory (as of 2023 knowledge cutoff)

Approximately 50% of the oxygen in Earth’s atmosphere is produced by phytoplankton, including cyanobacteria, through photosynthesis.

Source: National Geographic (as of 2023 knowledge cutoff)

Examples

Red Algae

Red algae (Rhodophyta) contain chloroplasts with unique pigments called phycoerythrins, which give them their characteristic red color and allow them to photosynthesize at greater depths in the ocean than most other algae.

Frequently Asked Questions

What is the role of chlorophyll in photosynthesis?

Chlorophyll is the primary photosynthetic pigment that absorbs light energy, particularly in the red and blue regions of the spectrum. This absorbed energy drives the light-dependent reactions of photosynthesis, ultimately leading to the production of ATP and NADPH.

Topics Covered

BotanyCell BiologyOrganellesPhotosynthesisPlant Physiology