UPSC MainsBOTANY-PAPER-II201810 Marks
Q1.

Organelles bound by membranes in eukaryotic cells have originated from endosymbiosis of prokaryotic cells." Explain this statement with the help of some suitable diagrams.

How to Approach

This question requires a detailed explanation of the endosymbiotic theory, a cornerstone of modern cell biology. The answer should begin by defining endosymbiosis and outlining the evidence supporting it. Focus on mitochondria and chloroplasts as prime examples. Diagrams are crucial for illustrating the process and structural similarities. The answer should also address the prokaryotic origins of these organelles, highlighting key features that support this claim. A clear, logical structure with appropriate headings and subheadings is essential.

Model Answer

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Introduction

The evolution of eukaryotic cells, characterized by their complex internal structure, represents a pivotal moment in the history of life. A central tenet explaining this complexity is the endosymbiotic theory, first proposed by Lynn Margulis in the 1960s. This theory posits that certain organelles within eukaryotic cells – specifically mitochondria and chloroplasts – were once free-living prokaryotic organisms that developed a symbiotic relationship with an ancestral eukaryotic cell. This symbiotic relationship eventually led to the integration of these prokaryotes into the host cell, resulting in the organelles we observe today. Understanding this process is fundamental to comprehending the origins of eukaryotic cell structure and function.

The Endosymbiotic Theory: A Detailed Explanation

Endosymbiosis, literally meaning “symbiosis within,” describes the process where one organism lives inside another, resulting in a mutually beneficial relationship. In the context of eukaryotic cell evolution, the theory proposes a series of endosymbiotic events. The most widely accepted scenario involves two major events: the engulfment of an aerobic bacterium (leading to mitochondria) and the engulfment of a photosynthetic bacterium (leading to chloroplasts).

Evidence Supporting Endosymbiosis

Several lines of evidence strongly support the endosymbiotic theory:

  • Structural Similarities: Mitochondria and chloroplasts resemble bacteria in several key aspects. They are similar in size to bacteria, have a double membrane (consistent with engulfment), and possess their own circular DNA, similar to bacterial chromosomes.
  • Independent Replication: These organelles replicate independently of the host cell through a process resembling binary fission, the method of reproduction used by bacteria.
  • Ribosomes: Mitochondria and chloroplasts contain ribosomes that are more similar to bacterial ribosomes (70S) than to the ribosomes found in the eukaryotic cytoplasm (80S).
  • Genetic Evidence: DNA sequencing reveals that mitochondrial and chloroplast DNA is more closely related to bacterial DNA than to the nuclear DNA of the eukaryotic cell.
  • Protein Synthesis: Protein synthesis within these organelles is inhibited by antibiotics that affect bacterial protein synthesis but not eukaryotic protein synthesis.

The Origin of Mitochondria

Mitochondria are believed to have originated from an aerobic alpha-proteobacterium. This bacterium was capable of efficient aerobic respiration, providing the host cell with a significant energy advantage. The host cell, in turn, provided the bacterium with a protected environment and nutrients. Over time, the bacterium lost its independence and evolved into the mitochondria we see today.

Mitochondrial Endosymbiosis

Diagram illustrating the endosymbiotic origin of mitochondria.

The Origin of Chloroplasts

Chloroplasts are thought to have originated from a photosynthetic cyanobacterium. This bacterium was capable of performing photosynthesis, providing the host cell with a source of organic molecules. Similar to the mitochondrial scenario, the host cell provided protection and nutrients, leading to a symbiotic relationship and eventual integration of the cyanobacterium into the host cell as a chloroplast.

Chloroplast Endosymbiosis

Diagram illustrating the endosymbiotic origin of chloroplasts.

Comparison of Prokaryotic and Organelle Characteristics

Characteristic Prokaryotic Cell Mitochondria/Chloroplasts Eukaryotic Cell (Cytoplasm)
DNA Circular, single chromosome Circular, multiple copies Linear, multiple chromosomes
Ribosomes 70S 70S 80S
Cell Wall Present (usually) Absent Present (in plants & fungi) or Absent (in animals)
Reproduction Binary Fission Binary Fission Mitosis/Meiosis

The presence of these prokaryotic-like features within mitochondria and chloroplasts provides compelling evidence for their endosymbiotic origins.

Conclusion

The endosymbiotic theory provides a robust explanation for the origin of mitochondria and chloroplasts, and consequently, the evolution of eukaryotic cells. The compelling evidence – structural similarities, independent replication, genetic relatedness, and biochemical characteristics – strongly supports the idea that these organelles were once free-living prokaryotes. This theory revolutionized our understanding of cell evolution and highlights the importance of symbiotic relationships in shaping the diversity of life on Earth. Further research continues to refine our understanding of the specific events and mechanisms involved in these ancient endosymbiotic partnerships.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Endosymbiosis
A symbiotic relationship in which one organism lives inside another.
Alpha-proteobacterium
A group of bacteria believed to be the ancestors of mitochondria.

Key Statistics

Approximately 90% of the proteins within mitochondria and chloroplasts are encoded by the nuclear genome of the host cell, reflecting the long-term integration of these organelles.

Source: Gray, M. W. (1999). Evolution of mitochondrial and chloroplast genomes. Annual Review of Genetics, 33(1), 577–607.

Mitochondria are found in nearly all eukaryotic cells, highlighting their fundamental role in cellular energy production. (Knowledge cutoff: 2023)

Source: Campbell Biology, 12th Edition

Examples

Secondary Endosymbiosis

Some eukaryotic algae have chloroplasts that originated from a secondary endosymbiotic event, where a eukaryotic cell engulfed another eukaryotic cell containing a chloroplast. This resulted in chloroplasts with four membranes.

Frequently Asked Questions

What is the significance of the double membrane surrounding mitochondria and chloroplasts?

The double membrane is a key piece of evidence supporting endosymbiosis. The inner membrane is believed to be derived from the original bacterial membrane, while the outer membrane is derived from the host cell's membrane during the engulfment process.

Topics Covered

BiologyCell BiologyCell StructureOrganellesEvolution