UPSC MainsBOTANY-PAPER-II201910 Marks
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Q2.

Citing example, discuss briefly the process of cytoplasmic inheritance and the genes involved.

How to Approach

This question requires a detailed understanding of cytoplasmic inheritance, differentiating it from Mendelian inheritance. The answer should define cytoplasmic inheritance, explain the organelles involved (chloroplasts and mitochondria), and provide specific examples illustrating the process and the genes involved. A structured approach focusing on the mechanism, examples, and genes will be effective. Mentioning the unique characteristics like maternal inheritance is crucial.

Model Answer

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Introduction

Cytoplasmic inheritance, also known as non-Mendelian inheritance, refers to the transmission of genes located outside the nucleus – specifically within organelles like mitochondria and chloroplasts. Unlike nuclear DNA which undergoes recombination and segregation during meiosis, cytoplasmic DNA is typically inherited maternally, meaning it’s passed down only from the mother. This inheritance pattern deviates from the principles established by Gregor Mendel, where genes are inherited equally from both parents. Understanding cytoplasmic inheritance is crucial for comprehending the genetic basis of certain plant traits and human diseases.

Mechanism of Cytoplasmic Inheritance

Cytoplasmic inheritance occurs due to the presence of DNA within organelles like mitochondria and chloroplasts. These organelles possess their own circular DNA, ribosomes, and can synthesize some of their own proteins. The key features of cytoplasmic inheritance are:

  • Maternal Inheritance: Most cytoplasmic genes are inherited solely from the mother because the egg cell contributes the majority of the cytoplasm to the zygote. Sperm cells contribute very little cytoplasm.
  • Non-Mendelian Segregation: The segregation patterns of cytoplasmic genes do not follow Mendel’s laws of independent assortment or dominance.
  • High Mutation Rate: Mitochondrial and chloroplast DNA have higher mutation rates compared to nuclear DNA, potentially leading to genetic diversity.
  • Heteroplasmy: Cells can contain a mixture of different mitochondrial DNA molecules (wild-type and mutated), leading to variable expression of traits.

Organelles Involved and Genes

Mitochondrial Inheritance

Mitochondria are responsible for cellular respiration and contain a relatively small genome (around 16,569 base pairs in humans). Mitochondrial genes encode for components of the electron transport chain, tRNA, and rRNA. Mutations in mitochondrial genes can cause a variety of human diseases, often affecting tissues with high energy demands like muscles and nerves.

  • Example: Human Mitochondrial Myopathies: Mutations in genes like MT-TL1 (tRNA leucine gene) can cause Mitochondrial Encephalomyopathy, Lactic Acidosis, and Stroke-like episodes (MELAS).
  • Genes Involved: MT-ATP6, MT-ATP8, MT-CO1, MT-CO2, MT-CO3, MT-ND1-6, MT-CYB (These genes encode for proteins involved in oxidative phosphorylation).

Chloroplast Inheritance

Chloroplasts are the sites of photosynthesis and are found in plant cells and algae. They contain a larger genome than mitochondria (around 120-160 kb). Chloroplast genes encode for proteins involved in photosynthesis, such as those in photosystems I and II.

  • Example: Four O’Clock Plant (Mirabilis jalapa): Karl Correns observed that leaf variegation (patches of white and green) in four o’clock plants was inherited maternally. If a variegated plant was crossed with a green plant, all the progeny inherited the variegated phenotype from the variegated parent. This is because the variegation is caused by mutations in chloroplast genes.
  • Genes Involved: Genes encoding for ribosomal proteins, photosystem proteins (psbA, psbC, rbcL), and other proteins involved in chloroplast function.

Specific Examples Illustrating Cytoplasmic Inheritance

Organism Trait Organelle Involved Gene(s) Involved Inheritance Pattern
Four O’Clock Plant (Mirabilis jalapa) Leaf Variegation Chloroplast psbA (D1 protein of photosystem II) Maternal
Yeast (Saccharomyces cerevisiae) Growth on specific media Mitochondria pet5 (cytochrome b gene) Maternal
Humans Leber’s Hereditary Optic Neuropathy (LHON) Mitochondria MT-ND4 Maternal

Male Sterility in Plants: Cytoplasmic male sterility (CMS) is a common phenomenon in many plant species, where plants are unable to produce functional pollen. This is often due to mutations in mitochondrial or chloroplast genes that disrupt pollen development. CMS is exploited in hybrid seed production.

Conclusion

Cytoplasmic inheritance represents a significant deviation from Mendelian genetics, highlighting the importance of extranuclear DNA in determining phenotypic traits. The maternal inheritance pattern and unique characteristics of organellar genomes contribute to its distinct segregation patterns. Understanding cytoplasmic inheritance is crucial not only for plant breeding and understanding plant traits but also for comprehending the genetic basis of human mitochondrial diseases. Further research into the complexities of heteroplasmy and the interplay between nuclear and cytoplasmic genes will continue to refine our understanding of this fascinating aspect of genetics.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Heteroplasmy
The presence of more than one type of mitochondrial DNA within a single cell. This can occur due to mutations or replication errors in mitochondrial DNA.
Cytoplasmic Male Sterility (CMS)
A phenomenon in plants where the plant is unable to produce functional pollen due to mutations in genes located in the cytoplasm (mitochondria or chloroplasts).

Key Statistics

Approximately 1 in 5,000 people are affected by mitochondrial diseases (as of 2023 data from the United Mitochondrial Disease Foundation).

Source: United Mitochondrial Disease Foundation

Mitochondrial DNA mutates at a rate approximately 10 times higher than nuclear DNA (based on knowledge cutoff 2023).

Source: Various genetics textbooks and research articles

Examples

Kearns-Sayre Syndrome (KSS)

A mitochondrial disease characterized by progressive external ophthalmoplegia, pigmentary retinopathy, and cardiac conduction defects. It is caused by large-scale deletions in mitochondrial DNA.

Frequently Asked Questions

Why is cytoplasmic inheritance usually maternal?

The egg cell contributes the vast majority of cytoplasm to the zygote, while the sperm contributes very little. Therefore, the organelles (mitochondria and chloroplasts) and their associated DNA are primarily inherited from the mother.

Topics Covered

BotanyGeneticsInheritance PatternsOrganelle GeneticsMitochondrial DNA