UPSC MainsBOTANY-PAPER-II201910 Marks
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Q12.

Describe the cellular structure and functions of Ribosome and Peroxisome.

How to Approach

This question requires a detailed understanding of two crucial cell organelles – Ribosomes and Peroxisomes. The answer should focus on their structural components, organization, and diverse functions within the cell. A comparative approach highlighting their differences alongside individual descriptions will be beneficial. Structure the answer by first introducing each organelle, then detailing its structure (including sub-units and components), followed by a comprehensive explanation of its functions. Include relevant examples to illustrate their importance.

Model Answer

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Introduction

Cells, the fundamental units of life, rely on specialized structures called organelles to perform specific functions. Ribosomes and Peroxisomes are two such organelles, vital for protein synthesis and detoxification, respectively. Ribosomes, ubiquitous in both prokaryotic and eukaryotic cells, are responsible for translating genetic code into proteins. Peroxisomes, found predominantly in eukaryotic cells, play a critical role in various metabolic processes, including fatty acid oxidation and detoxification of harmful substances. Understanding their structure and function is crucial for comprehending cellular processes and their implications in health and disease.

Ribosomes: Structure and Function

Ribosomes are complex molecular machines responsible for protein synthesis, a process known as translation. They are not membrane-bound organelles, hence are not considered true organelles in the same sense as mitochondria or chloroplasts.

Structure

  • Subunits: Ribosomes are composed of two subunits – a large subunit and a small subunit. These subunits are made of ribosomal RNA (rRNA) and proteins.
  • Prokaryotic Ribosomes: Prokaryotic ribosomes are 70S in size, consisting of a 50S large subunit and a 30S small subunit.
  • Eukaryotic Ribosomes: Eukaryotic ribosomes are 80S in size, comprising a 60S large subunit and a 40S small subunit.
  • rRNA molecules: The rRNA molecules within each subunit play a catalytic role in peptide bond formation.
  • Location: Ribosomes can be found free-floating in the cytoplasm or bound to the endoplasmic reticulum (forming rough ER).

Functions

  • Protein Synthesis: The primary function of ribosomes is to translate mRNA into proteins. tRNA molecules bring amino acids to the ribosome, where they are linked together according to the mRNA sequence.
  • Decoding Genetic Information: Ribosomes accurately decode the genetic information encoded in mRNA.
  • Peptide Bond Formation: Ribosomes catalyze the formation of peptide bonds between amino acids.
  • Regulation of Gene Expression: Ribosomes can also play a role in regulating gene expression.

Peroxisomes: Structure and Function

Peroxisomes are single-membrane-bound organelles found in nearly all eukaryotic cells. They are involved in a variety of metabolic processes, including oxidation reactions that produce hydrogen peroxide (H2O2) as a byproduct, hence the name "peroxisome."

Structure

  • Membrane: Peroxisomes are enclosed by a single phospholipid bilayer membrane.
  • Matrix: The interior of the peroxisome contains a dense matrix with various enzymes.
  • Crystalline Core: Some peroxisomes contain a crystalline core composed of urate oxidase, an enzyme involved in purine metabolism.
  • Enzymes: Peroxisomes contain a diverse array of enzymes, including catalase, oxidases, and peroxidases.

Functions

  • Fatty Acid Oxidation: Peroxisomes break down very long-chain fatty acids through beta-oxidation, generating acetyl-CoA.
  • Detoxification: Peroxisomes detoxify harmful substances, such as alcohol and formaldehyde, using catalase to decompose hydrogen peroxide into water and oxygen.
  • Synthesis of Plasmalogens: Peroxisomes synthesize plasmalogens, a class of phospholipids essential for brain and heart function.
  • Photorespiration: In plant cells, peroxisomes play a role in photorespiration, a metabolic pathway that occurs in chloroplasts, peroxisomes, and mitochondria.
  • Cholesterol Metabolism: Peroxisomes are involved in cholesterol metabolism in animal cells.

Comparative Table: Ribosomes vs. Peroxisomes

Feature Ribosomes Peroxisomes
Membrane Bound No Yes (Single Membrane)
Primary Function Protein Synthesis Detoxification & Metabolic Reactions
Subunits Large & Small (50S/30S or 60S/40S) None (Single Compartment)
Key Enzymes Peptidyl Transferase Catalase, Oxidases
Location Cytoplasm, Rough ER Cytoplasm

Conclusion

In conclusion, ribosomes and peroxisomes, though distinct in structure and function, are both essential organelles for cellular survival. Ribosomes are the protein synthesis machinery, translating genetic information into functional proteins, while peroxisomes are metabolic hubs involved in detoxification, fatty acid oxidation, and other crucial processes. Their coordinated activity ensures proper cellular function and homeostasis. Further research into these organelles continues to reveal their complex roles in health and disease, offering potential targets for therapeutic interventions.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Translation
The process by which the genetic code in mRNA is decoded to produce a specific protein.
Beta-oxidation
A metabolic process that breaks down fatty acids into acetyl-CoA, which can then be used for energy production.

Key Statistics

Approximately 80% of the cellular RNA is ribosomal RNA (rRNA).

Source: Alberts et al., Molecular Biology of the Cell, 6th edition (2015)

Peroxisomes constitute approximately 0.5-1% of the total liver cell volume.

Source: Lodish et al., Molecular Cell Biology, 7th edition (2008)

Examples

Zellweger Syndrome

A genetic disorder caused by defects in peroxisome biogenesis, leading to the accumulation of very long-chain fatty acids and severe neurological problems. This highlights the critical role of functional peroxisomes.

Frequently Asked Questions

What happens if a cell lacks functional ribosomes?

A cell lacking functional ribosomes would be unable to synthesize proteins, leading to a complete halt in cellular processes and ultimately cell death.

Topics Covered

BiologyCell BiologyCell StructureOrganellesProtein Synthesis