UPSC MainsZOOLOGY-PAPER-II201910 Marks
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Q5.

Explain the Stanley Miller's experiment related to origin of life.

How to Approach

This question requires a detailed explanation of Stanley Miller’s experiment, a landmark study in understanding the origin of life. The answer should focus on the experimental setup, the process, the results, and its significance. It’s crucial to explain the prevailing hypothesis at the time (Oparin-Haldane) and how Miller’s experiment provided evidence supporting it. Structure the answer chronologically, starting with the background, then detailing the experiment, and finally discussing its implications and limitations.

Model Answer

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Introduction

The question of life’s origin has captivated scientists for centuries. In the early 20th century, Alexander Oparin and J.B.S. Haldane independently proposed the ‘primordial soup’ hypothesis, suggesting that life arose gradually from inorganic molecules in a reducing atmosphere. However, this remained a theoretical framework until Stanley Miller, guided by Harold Urey, conducted a groundbreaking experiment in 1953. This experiment aimed to simulate the conditions thought to prevail on early Earth and test the possibility of abiotic synthesis of organic molecules – the building blocks of life. This answer will detail the methodology, results, and significance of Miller’s experiment.

The Oparin-Haldane Hypothesis: Setting the Stage

Before delving into Miller’s experiment, understanding the prevailing theory is crucial. The Oparin-Haldane hypothesis (1920s) posited that early Earth had a reducing atmosphere rich in gases like methane, ammonia, water vapor, and hydrogen. Energy sources like lightning, UV radiation, and volcanic activity would have provided the energy needed to form simple organic molecules from these inorganic precursors. These molecules would accumulate in the oceans, forming a ‘primordial soup’ where further reactions could lead to more complex structures and eventually, life.

The Stanley Miller-Harold Urey Experiment: A Detailed Account

In 1953, Stanley Miller, a graduate student under Harold Urey at the University of Chicago, designed an experiment to test the Oparin-Haldane hypothesis. The experimental setup consisted of a closed system of glass flasks connected in a loop.

  • Flask 1: Contained boiling water to simulate the early Earth’s oceans.
  • Flask 2: Contained the ‘atmosphere’ – a mixture of gases believed to be present on early Earth: methane (CH4), ammonia (NH3), water vapor (H2O), and hydrogen (H2). Oxygen was deliberately excluded as it’s a highly reactive gas that would inhibit the formation of organic molecules.
  • Spark Chamber: Two electrodes were used to generate sparks, simulating lightning.
  • Condenser: A cold surface cooled the gases, causing them to condense into a liquid.
  • Collection Trap: The condensed liquid accumulated in a trap, simulating the early oceans.

The experiment ran continuously for a week. At the end of the week, the liquid in the collection trap was analyzed. The results were remarkable.

Results and Significance

The analysis revealed that several organic molecules, including amino acids (glycine, alanine, aspartic acid), had formed. These amino acids are the building blocks of proteins, essential for life. Other organic compounds like sugars and lipids were also detected in smaller quantities.

The experiment demonstrated that organic molecules could indeed be synthesized abiotically under conditions thought to resemble early Earth. This provided strong support for the Oparin-Haldane hypothesis and the idea that life could have originated from non-living matter. It was a pivotal moment in the study of the origin of life.

Limitations and Subsequent Research

While groundbreaking, Miller’s experiment wasn’t without limitations. The initial atmospheric composition used (highly reducing) is now believed to be less accurate. Geological evidence suggests that the early Earth’s atmosphere may have been less reducing and contained more carbon dioxide and nitrogen.

Subsequent experiments, using different gas mixtures and energy sources, have also yielded organic molecules. Furthermore, the formation of polymers (like proteins and nucleic acids) from these monomers remains a challenge. Research continues to explore alternative environments for the origin of life, such as hydrothermal vents and meteorites. Miller himself conducted further experiments later in his career, refining the initial setup and exploring different atmospheric compositions.

The Urey-Miller Experiment and RNA World Hypothesis

The Urey-Miller experiment also indirectly supported the RNA world hypothesis. While the experiment produced amino acids, the formation of RNA nucleotides was also demonstrated in later variations of the experiment. The RNA world hypothesis suggests that RNA, not DNA, was the primary genetic material in early life, possessing both genetic information storage and catalytic capabilities.

Conclusion

Stanley Miller’s experiment remains a cornerstone in the study of the origin of life. It provided the first experimental evidence supporting the abiotic synthesis of organic molecules, bolstering the Oparin-Haldane hypothesis. While our understanding of early Earth’s conditions has evolved, and challenges remain in explaining the transition from simple molecules to complex life forms, Miller’s work laid the foundation for future research and continues to inspire scientists exploring the mysteries of life’s beginnings. The experiment highlighted the potential for life to arise through natural processes, even in the absence of biological intervention.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Abiogenesis
The natural process by which life has arisen from non-living matter, such as simple organic compounds.
Reducing Atmosphere
An atmosphere lacking in free oxygen, and rich in gases like methane, ammonia, and hydrogen. Such an atmosphere favors the formation of organic molecules.

Key Statistics

The original Miller-Urey experiment yielded approximately 15 different amino acids, some of which are essential for protein synthesis.

Source: Miller, S. L. (1953). A production of amino acids under possible early earth conditions. *Science*, *117*(3043), 533–541.

Approximately 85% of the carbon in the Murchison meteorite was found to be in organic compounds.

Source: Cronin, J. R., & Chang, S. (1993). Organic matter in meteorites: molecular inventory and marker molecules. *Van Nostrand Reinhold*.

Examples

Hydrothermal Vents

Hydrothermal vents, found in deep-sea environments, release chemicals from Earth’s interior. These vents provide energy and chemical building blocks, and are considered potential sites for the origin of life, offering an alternative to the ‘primordial soup’ scenario.

Frequently Asked Questions

Did the Miller-Urey experiment create life?

No, the experiment did not create life. It only demonstrated the abiotic synthesis of organic molecules – the building blocks of life. The formation of self-replicating systems and the emergence of cellular structures are still areas of active research.

Topics Covered

BiologyEvolutionBiochemistryOrigin of LifeAbiogenesisPrebiotic Chemistry