UPSC MainsBOTANY-PAPER-I202015 Marks
Q8.

Describe lytic and lysogenic cycle of viruses.

How to Approach

This question requires a detailed understanding of viral replication strategies. The answer should clearly differentiate between the lytic and lysogenic cycles, outlining the steps involved in each. A comparative table highlighting the key differences would be beneficial. Focus on the mechanisms, outcomes, and implications of each cycle. The answer should demonstrate a strong grasp of microbiology principles.

Model Answer

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Introduction

Viruses are obligate intracellular parasites, meaning they can only replicate inside a host cell. Their replication strategies are diverse, but broadly categorized into two main cycles: the lytic cycle and the lysogenic cycle. These cycles represent different approaches viruses take to utilize the host cell machinery for their own propagation. Understanding these cycles is crucial for comprehending viral pathogenesis, developing antiviral therapies, and controlling viral infections. The choice between these cycles often depends on the virus type and the host cell's condition.

Lytic Cycle

The lytic cycle is a rapid and destructive process resulting in the immediate lysis (rupture) of the host cell. It’s characterized by active viral replication and the production of numerous viral particles.

  • Attachment (Adsorption): The virus attaches to specific receptors on the host cell surface.
  • Penetration (Entry): The viral genome enters the host cell. This can occur through various mechanisms, including direct injection, endocytosis, or membrane fusion.
  • Replication: The viral genome takes control of the host cell's machinery, directing it to replicate viral nucleic acids and synthesize viral proteins.
  • Assembly (Maturation): Newly synthesized viral components (nucleic acids and proteins) are assembled into new viral particles (virions).
  • Release (Lysis): The host cell lyses, releasing the newly formed virions, which can then infect other cells. This lysis is often facilitated by viral enzymes like lysozyme.

Examples of viruses that primarily utilize the lytic cycle include bacteriophage T4 and influenza virus.

Lysogenic Cycle

The lysogenic cycle is a more subtle and prolonged process where the viral genome integrates into the host cell's genome, becoming a prophage (in bacteria) or provirus (in eukaryotes). The host cell is not immediately destroyed.

  • Attachment & Penetration: Similar to the lytic cycle, the virus attaches to and enters the host cell.
  • Integration: The viral genome integrates into the host cell's DNA. In bacteria, this is often site-specific integration.
  • Prophage/Provirus Replication: The integrated viral genome (prophage/provirus) is replicated along with the host cell's DNA during cell division. Each daughter cell receives a copy of the viral genome.
  • Induction: Under certain stress conditions (e.g., UV radiation, nutrient deprivation), the prophage/provirus can excise itself from the host genome and enter the lytic cycle.

Examples of viruses that can undergo lysogeny include bacteriophage lambda (λ) and HIV.

Comparison of Lytic and Lysogenic Cycles

Feature Lytic Cycle Lysogenic Cycle
Host Cell Survival Host cell is destroyed (lysed) Host cell survives (initially)
Viral Replication Rapid and immediate Delayed; viral genome replicates with host DNA
Viral Genome Replicates independently Integrates into host genome
Progeny Virus Production Large number of virions released quickly No immediate virion production; potential for later lytic cycle
Induction Not applicable Can be induced to enter lytic cycle

Temperate Viruses

Viruses capable of undergoing both lytic and lysogenic cycles are called temperate viruses. The decision between the two cycles is often influenced by environmental factors and the physiological state of the host cell.

Conclusion

In conclusion, the lytic and lysogenic cycles represent distinct strategies employed by viruses to replicate and propagate. The lytic cycle is characterized by rapid replication and host cell destruction, while the lysogenic cycle involves integration into the host genome and delayed replication. Understanding these cycles is fundamental to comprehending viral infections and developing effective antiviral strategies. The ability of some viruses to switch between these cycles adds complexity to their pathogenesis and control.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Virion
A complete, infectious virus particle, consisting of the viral genome enclosed in a protein coat (capsid).
Prophage
The viral genome integrated into a bacterial chromosome during the lysogenic cycle.

Key Statistics

Approximately 30% of bacterial genes are thought to have originated from viruses through horizontal gene transfer during lysogeny.

Source: Hendrix, R. E., et al. (2016). Evolution of microbial genomes through viral transfer.

It is estimated that approximately 8% of the human genome consists of endogenous retroviral sequences, remnants of ancient retroviral infections that integrated into the germline.

Source: Belfrage, P., & Andersson, R. (2008). The human genome and endogenous retroviruses.

Examples

Bacteriophage Lambda (λ)

Bacteriophage lambda infects *E. coli* and can enter either the lytic or lysogenic cycle. The decision is regulated by the cI repressor protein, which promotes lysogeny by suppressing the expression of lytic genes.

Frequently Asked Questions

What is the significance of the lysogenic cycle for viral evolution?

The lysogenic cycle allows viruses to persist within a host population without causing immediate harm. During this time, the viral genome can undergo mutations and acquire new genes from the host, contributing to viral evolution and adaptation.

Topics Covered

BotanyMicrobiologyVirusesReplicationHost-Virus Interaction