UPSC MainsBOTANY-PAPER-II20207 Marks
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Q8.

Polyploidy in plant breeding

How to Approach

This question requires a detailed understanding of polyploidy, its mechanisms, types, and applications in plant breeding. The answer should begin with a clear definition of polyploidy and its significance. It should then delve into the different types of polyploidy (autopolyploidy and allopolyploidy), their induction methods, and the advantages and disadvantages associated with each. Finally, the answer must highlight the role of polyploidy in crop improvement, providing specific examples of polyploid crops and their benefits. A structured approach using headings and subheadings will enhance clarity.

Model Answer

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Introduction

Polyploidy, derived from the Greek words ‘poly’ meaning many and ‘ploid’ meaning units, refers to the condition where a cell or organism possesses more than two complete sets of chromosomes. While most animals maintain a diploid state (2n), polyploidy is surprisingly common in plants, estimated to be present in 30-80% of flowering plant species. This genomic alteration has played a crucial role in plant evolution and has been extensively exploited in plant breeding to enhance desirable traits like size, yield, and disease resistance. Understanding the mechanisms and implications of polyploidy is therefore fundamental to modern agricultural practices.

Understanding Polyploidy

Polyploidy arises due to errors during cell division, specifically during meiosis or mitosis, leading to the duplication of the entire genome. It’s a significant evolutionary force in plants, contributing to speciation and adaptation. Polyploidy can be classified into two main types:

1. Autopolyploidy

Autopolyploidy results from the duplication of chromosomes within a single species. It occurs when there is a failure of homologous chromosomes to separate during meiosis, leading to gametes with doubled chromosome numbers. These gametes, upon fertilization, produce offspring with more than two sets of chromosomes derived from the same species. For example, a diploid (2n=6) plant producing tetraploid (4n=12) offspring.

  • Induction: Can be induced by colchicine, a chemical that inhibits spindle fiber formation during cell division.
  • Advantages: Increased vigor, larger size, and potentially improved yield.
  • Disadvantages: Reduced fertility due to irregular meiosis, potential for chromosome instability.

2. Allopolyploidy

Allopolyploidy arises from the combination of chromosome sets from two different species through hybridization, followed by chromosome doubling. This often involves the pairing of homologous chromosomes from both parental species, resulting in a stable polyploid hybrid. Allopolyploidy is particularly important in the origin of new plant species.

  • Induction: Hybridization followed by chromosome doubling (often induced by colchicine).
  • Advantages: Combines desirable traits from two different species, often resulting in increased vigor and fertility.
  • Disadvantages: Initial hybridization may be difficult, potential for genetic incompatibility.

Methods of Inducing Polyploidy

Several methods are employed to induce polyploidy in plants:

  • Colchicine Treatment: The most common method, colchicine disrupts microtubule formation, preventing chromosome separation during cell division.
  • Cold Treatment: Exposure to low temperatures can also induce polyploidy by disrupting meiosis.
  • In vitro Techniques: Tissue culture techniques can be used to induce polyploidy in meristematic tissues.

Applications of Polyploidy in Plant Breeding

Polyploidy has been extensively used in plant breeding to improve crop characteristics:

  • Increased Yield: Polyploid plants often exhibit larger cell size and increased biomass, leading to higher yields.
  • Enhanced Size and Vigor: Polyploidy can result in larger fruits, flowers, and overall plant size.
  • Improved Disease Resistance: Polyploidy can sometimes enhance resistance to certain diseases.
  • Seedlessness: Triploid plants (3n) are often sterile and produce seedless fruits (e.g., seedless watermelons).

Examples of Polyploid Crops

Crop Ploidy Level Benefits
Wheat Hexaploid (6n) High yield, adaptability to various climates
Potato Tetraploid (4n) Increased tuber size and yield
Sugarcane Polyploid (variable) High sucrose content, vigorous growth
Banana Triploid (3n) Seedlessness, convenient for consumption
Strawberry Octoploid (8n) Large fruit size, improved flavor

Recent advancements in genomics and molecular breeding are allowing for more precise manipulation of polyploid genomes, leading to the development of even more improved crop varieties. Genome editing technologies like CRISPR-Cas9 are being explored to correct undesirable traits associated with polyploidy, such as reduced fertility.

Conclusion

Polyploidy is a powerful tool in plant breeding, offering significant advantages in terms of yield, size, and adaptability. While challenges related to fertility and genetic instability exist, advancements in breeding techniques and genomic technologies are continually expanding the potential of polyploidy for crop improvement. Continued research into the mechanisms governing polyploid genome expression and stability will be crucial for maximizing its benefits in ensuring global food security.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Genome
The complete set of genetic instructions in an organism. It includes all of its genes and their associated DNA.
Haploid
An organism or cell having only one set of chromosomes.

Key Statistics

Approximately 70% of flowering plants have undergone at least one round of whole-genome duplication in their evolutionary history.

Source: Soltis PS, Soltis DE. Polyploidy: the key to flowering plant evolution. Annu Rev Plant Biol. 2000;51:585-609.

The global market for seedless fruit, largely enabled by polyploidy, is estimated to be worth over $10 billion annually (as of 2023).

Source: Market research reports (knowledge cutoff 2024)

Examples

Raphanobrassica

Raphanobrassica is a classic example of an allopolyploid, created by crossing a radish (Raphanus sativus) and a cabbage (Brassica oleracea). It exhibits characteristics of both parents, such as edible roots and leaves.

Frequently Asked Questions

Is polyploidy always beneficial?

No, polyploidy isn't always beneficial. While it can lead to increased vigor and yield, it can also result in reduced fertility, slower growth, and genetic instability. The effects of polyploidy depend on the specific species and the type of polyploidy.

Topics Covered

BiologyPlant ScienceGeneticsPlant BreedingGenome EvolutionCrop Science