UPSC MainsBOTANY-PAPER-II202210 Marks150 Words
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Q17.

Photophosphorylation

How to Approach

This question requires a detailed explanation of photophosphorylation, a crucial process in photosynthesis. The answer should cover the different types – cyclic and non-cyclic – outlining their mechanisms, electron transport chains, and end products. A comparative table highlighting the differences between the two would be beneficial. Focus on the role of photosystems I and II, and the generation of ATP and NADPH. The answer should demonstrate a clear understanding of the biochemical pathways involved.

Model Answer

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Introduction

Photophosphorylation is the process of transferring energy from light into chemical energy in the form of ATP (adenosine triphosphate). It is a vital step in photosynthesis, occurring in the thylakoid membranes within chloroplasts. This process utilizes light energy to drive the phosphorylation of ADP (adenosine diphosphate) to ATP, providing the energy required for the subsequent carbon fixation reactions in the Calvin cycle. Understanding photophosphorylation is fundamental to comprehending how plants convert light energy into usable chemical energy, sustaining life on Earth.

Photophosphorylation: A Detailed Overview

Photophosphorylation is the process of using light energy to generate ATP. It occurs in the thylakoid membranes of chloroplasts and is essential for the light-dependent reactions of photosynthesis. There are two main types of photophosphorylation: non-cyclic and cyclic.

Non-Cyclic Photophosphorylation

Non-cyclic photophosphorylation involves both Photosystem II (PSII) and Photosystem I (PSI). Here’s a step-by-step breakdown:

  • Light Absorption: PSII absorbs light energy, exciting electrons to a higher energy level.
  • Water Splitting: To replenish the electrons lost by PSII, water molecules are split (photolysis) into electrons, protons (H+), and oxygen (O2). This is where oxygen, a byproduct of photosynthesis, is released.
  • Electron Transport Chain: The excited electrons from PSII are passed along an electron transport chain (ETC) to PSI. As electrons move down the ETC, energy is released, which is used to pump protons (H+) from the stroma into the thylakoid lumen, creating a proton gradient.
  • ATP Synthesis: The proton gradient drives ATP synthase, an enzyme that phosphorylates ADP to ATP (chemiosmosis).
  • PSI and NADPH Formation: PSI also absorbs light energy, re-exciting electrons. These electrons are then used to reduce NADP+ to NADPH.

End Products: ATP, NADPH, and O2.

Cyclic Photophosphorylation

Cyclic photophosphorylation involves only PSI. It occurs when the ratio of NADPH to NADP+ is high, indicating a need for more ATP but less reducing power.

  • Light Absorption: PSI absorbs light energy, exciting electrons.
  • Electron Transport Chain: The excited electrons are passed along a cyclic electron transport chain, returning to PSI.
  • ATP Synthesis: As electrons move through the ETC, protons are pumped into the thylakoid lumen, creating a proton gradient that drives ATP synthesis.

End Product: ATP only. No NADPH or O2 is produced.

Comparison of Cyclic and Non-Cyclic Photophosphorylation

Feature Non-Cyclic Photophosphorylation Cyclic Photophosphorylation
Photosystems Involved PSII and PSI PSI only
Water Splitting Occurs Does not occur
Oxygen Evolution Yes No
NADPH Production Yes No
ATP Production Yes Yes
Electron Flow Linear Cyclic

Z-Scheme of Photosynthesis

The Z-scheme describes the flow of electrons during non-cyclic photophosphorylation. It illustrates the redox potential changes of electron carriers as electrons move from water to NADP+, resembling a 'Z' shape on a redox potential diagram. This scheme highlights the crucial role of PSII and PSI in capturing light energy and driving electron transport.

Conclusion

Photophosphorylation is a cornerstone of photosynthesis, enabling plants to convert light energy into the chemical energy necessary for life. The two types, cyclic and non-cyclic, operate under different conditions to optimize ATP and NADPH production. Understanding the intricacies of these processes, including the electron transport chains and the role of photosystems, is crucial for comprehending the broader context of plant physiology and global carbon cycling. Further research into enhancing photophosphorylation efficiency could have significant implications for improving crop yields and developing sustainable energy solutions.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

ATP Synthase
An enzyme that catalyzes the synthesis of ATP from ADP and inorganic phosphate, utilizing the proton gradient across the thylakoid membrane.
Chemiosmosis
The movement of ions across a selectively permeable membrane, down their electrochemical gradient. This process is used to generate ATP during photophosphorylation and cellular respiration.

Key Statistics

Approximately 30% of the energy captured by photosynthesis is converted into ATP and NADPH during photophosphorylation.

Source: Raven, P. H., Evert, R. F., & Eichhorn, S. E. (2013). Biology of Plants (8th ed.). W. H. Freeman and Company.

The efficiency of light conversion in natural photosynthesis is around 3-6%, while theoretical maximum efficiency is around 40-50%.

Source: Taiz, L., & Zeiger, E. (2010). Plant Physiology and Development (6th ed.). Sinauer Associates.

Examples

C4 Plants

C4 plants, like maize and sugarcane, employ a spatial separation of initial CO2 fixation and the Calvin cycle to minimize photorespiration. They often exhibit enhanced cyclic photophosphorylation to meet the increased ATP demands of the C4 pathway.

Frequently Asked Questions

What is the role of plastoquinone in photophosphorylation?

Plastoquinone (PQ) is a mobile electron carrier in the electron transport chain. It accepts electrons from PSII and transports them to the cytochrome b6f complex, contributing to the proton gradient formation.

Topics Covered

BiologyBotanyPhotosynthesisPlant PhysiologyEnergy Production