UPSC MainsBOTANY-PAPER-II202310 Marks150 Words
Q1.

Cell-cell adhesion mechanism

How to Approach

This question requires a detailed understanding of the mechanisms governing cell-cell adhesion. The answer should cover the major types of cell adhesion molecules (CAMs), their structural features, the types of junctions they form, and the significance of these adhesions in biological processes. A structured approach, categorizing CAMs and junction types, will be beneficial. Focus on providing specific examples and highlighting the importance of these mechanisms in development and tissue function.

Model Answer

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Introduction

Cell-cell adhesion is a crucial process in multicellular organisms, enabling cells to interact and form tissues and organs. It’s fundamental for development, immunity, wound healing, and maintaining tissue integrity. These interactions are mediated by a diverse group of cell surface proteins called Cell Adhesion Molecules (CAMs). Disruptions in cell adhesion are often implicated in diseases like cancer, where cells lose their normal adhesive properties and metastasize. Understanding these mechanisms is vital for comprehending both normal physiology and pathological conditions.

Types of Cell Adhesion Molecules (CAMs)

CAMs can be broadly categorized into four major families:

  • Cadherins: Calcium-dependent adhesion molecules, crucial for tissue formation and maintenance. They mediate homophilic interactions (binding to the same type of cadherin on another cell). Different types of cadherins (E-, N-, P-, R-cadherins) are expressed in different tissues, contributing to tissue specificity.
  • Immunoglobulin Superfamily (IgSF) CAMs: Characterized by immunoglobulin-like domains. Examples include NCAM (Neural Cell Adhesion Molecule) and ICAM (Intercellular Adhesion Molecule). They can mediate both homophilic and heterophilic interactions and play roles in immune responses and neuronal development.
  • Integrins: Heterodimeric transmembrane receptors that bind to extracellular matrix (ECM) proteins like fibronectin and collagen. They mediate cell-ECM adhesion and also participate in cell-cell adhesion through interactions with ligands on other cells. Integrins are involved in cell signaling, migration, and differentiation.
  • Selectins: Bind to carbohydrate moieties on other cells. They are expressed on endothelial cells and leukocytes and play a critical role in leukocyte trafficking during inflammation.

Types of Cell-Cell Junctions

CAMs function to form various types of cell-cell junctions, each with distinct structural and functional characteristics:

  • Tight Junctions: Form a seal between cells, preventing the passage of molecules between them. They are crucial for maintaining epithelial barrier function. Proteins like occludin and claudins are key components.
  • Adherens Junctions: Mediate strong cell-cell adhesion through cadherins. They are linked to the actin cytoskeleton, providing mechanical strength and contributing to tissue shape.
  • Desmosomes: Provide even stronger adhesion than adherens junctions, utilizing cadherins (desmoglein and desmocollin) and intermediate filaments (keratin). They are abundant in tissues subjected to mechanical stress, like skin and heart muscle.
  • Gap Junctions: Form channels between cells, allowing direct communication through the passage of small molecules and ions. They are formed by connexin proteins and are important for coordinating cellular activities.

Significance of Cell-Cell Adhesion

Cell-cell adhesion is essential for numerous biological processes:

  • Development: Crucial for cell sorting, tissue morphogenesis, and organ formation.
  • Immune Response: Mediates interactions between immune cells and target cells, facilitating immune surveillance and effector functions.
  • Wound Healing: Essential for cell migration and tissue repair.
  • Cancer Metastasis: Loss of cell adhesion is a hallmark of cancer metastasis, allowing cancer cells to detach from the primary tumor and invade surrounding tissues.
Junction Type CAM Involved Cytoskeletal Linkage Function
Tight Junctions Occludin, Claudins None Barrier function, preventing paracellular transport
Adherens Junctions Cadherins Actin Strong adhesion, tissue shape
Desmosomes Desmoglein, Desmocollin Intermediate Filaments (Keratin) Very strong adhesion, mechanical strength
Gap Junctions Connexins None Direct cell-cell communication

Conclusion

Cell-cell adhesion is a complex and dynamic process vital for maintaining tissue integrity and orchestrating numerous biological functions. The diverse array of CAMs and junction types allows for precise control over cell interactions, contributing to both normal development and disease pathogenesis. Further research into these mechanisms holds promise for developing novel therapeutic strategies for conditions like cancer and autoimmune diseases, where disruptions in cell adhesion play a significant role.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Homophilic Interaction
A type of molecular interaction where identical molecules bind to each other. Cadherins are a prime example, binding to the same cadherin type on adjacent cells.
Heterophilic Interaction
A type of molecular interaction where different molecules bind to each other. Integrins interacting with ECM proteins exemplify this.

Key Statistics

Approximately 20% of all human genes encode for proteins involved in cell adhesion and the extracellular matrix.

Source: Alberts et al., Molecular Biology of the Cell, 6th edition (2015)

Studies suggest that approximately 90% of human cancers exhibit altered expression of cadherins, contributing to their metastatic potential.

Source: Thiery JP, et al. Epithelial-mesenchymal transition in development and disease. Cell. 2009;139(3):581-92.

Examples

Epithelial Polarity

Tight junctions in epithelial cells establish apical-basolateral polarity, crucial for directional transport of nutrients and waste products. This polarity is essential for the proper functioning of organs like the intestine and kidney.

Frequently Asked Questions

What is the role of calcium in cadherin function?

Calcium ions bind to specific domains within cadherin molecules, inducing a conformational change that allows them to dimerize and engage in homophilic interactions. Without calcium, cadherins lose their adhesive properties.

Topics Covered

BiologyCell BiologyCell StructureCell CommunicationTissue Biology