UPSC MainsBOTANY-PAPER-II202310 Marks150 Words
Q2.

Structure and functions of cytoskeleton

How to Approach

This question requires a detailed understanding of the cytoskeleton – its components, structure, and diverse functions within a cell. The answer should begin by defining the cytoskeleton and its importance. Then, it should systematically discuss the three main types of filaments: microtubules, actin filaments, and intermediate filaments, detailing their structure, protein composition, and specific roles in cellular processes. Emphasis should be placed on how these filaments interact and contribute to cell shape, movement, and intracellular transport. A concise and organized approach, utilizing headings and subheadings, will maximize clarity and impact.

Model Answer

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Introduction

The cytoskeleton is a complex, dynamic network of protein filaments extending throughout the cytoplasm of eukaryotic cells. It’s not a static structure, but rather a highly organized and adaptable framework crucial for maintaining cell shape, enabling cellular motion, and facilitating intracellular transport. Discovered in the late 19th century, its significance became increasingly apparent with advancements in microscopy and cell biology. Understanding the cytoskeleton is fundamental to comprehending cellular processes like cell division, muscle contraction, and nerve impulse transmission. Its disruption is often linked to various diseases, highlighting its vital role in maintaining cellular health.

Components of the Cytoskeleton

The cytoskeleton is primarily composed of three main types of filaments:

1. Microtubules

Microtubules are hollow tubes composed of α- and β-tubulin dimers. They are the largest of the three components and are crucial for several cellular functions:

  • Structure: Approximately 25nm in diameter, formed by polymerization of tubulin dimers.
  • Functions:
    • Cell Division: Form the mitotic spindle, separating chromosomes during mitosis and meiosis.
    • Intracellular Transport: Serve as tracks for motor proteins (kinesin and dynein) to transport vesicles and organelles.
    • Cell Shape & Support: Contribute to maintaining cell shape and resisting compression.
    • Cilia and Flagella: Form the core structure of cilia and flagella, enabling cell motility.

2. Actin Filaments (Microfilaments)

Actin filaments are the most abundant cytoskeletal component and are involved in cell shape, movement, and muscle contraction.

  • Structure: Approximately 7nm in diameter, formed by polymerization of globular actin (G-actin) monomers into filamentous actin (F-actin).
  • Functions:
    • Cell Motility: Drive cell crawling, migration, and changes in cell shape.
    • Muscle Contraction: Interact with myosin to generate force for muscle contraction.
    • Cytokinesis: Form the contractile ring that divides the cytoplasm during cell division.
    • Microvilli: Provide structural support for microvilli, increasing surface area for absorption.

3. Intermediate Filaments

Intermediate filaments provide mechanical strength and stability to cells and tissues.

  • Structure: Approximately 10nm in diameter, composed of various proteins depending on the cell type (e.g., keratin, vimentin, lamins). They are more stable than microtubules and actin filaments.
  • Functions:
    • Mechanical Strength: Resist tensile forces and maintain cell shape.
    • Nuclear Lamina: Form the nuclear lamina, providing structural support to the nucleus.
    • Cell-Cell Junctions: Contribute to the formation of desmosomes and hemidesmosomes.

Cytoskeletal Dynamics and Interactions

The cytoskeleton is a dynamic structure, constantly undergoing assembly and disassembly. This dynamic instability is regulated by various factors, including signaling pathways and motor proteins. The three filament systems interact with each other to coordinate cellular processes. For example, microtubules can interact with actin filaments to regulate cell polarity and migration. Intermediate filaments provide a stable scaffold for the other two components.

Filament Type Diameter (nm) Protein Subunit Primary Function
Microtubules 25 α- and β-tubulin Intracellular transport, cell division, cell shape
Actin Filaments 7 G-actin Cell motility, muscle contraction, cytokinesis
Intermediate Filaments 10 Keratin, vimentin, lamins Mechanical strength, nuclear support

Conclusion

In conclusion, the cytoskeleton is a remarkably versatile and essential component of eukaryotic cells. Its dynamic nature and intricate interplay between microtubules, actin filaments, and intermediate filaments enable cells to perform a wide range of functions, from maintaining shape and facilitating movement to orchestrating cell division and intracellular transport. Disruptions in cytoskeletal function are implicated in numerous diseases, underscoring its critical role in maintaining cellular health and organismal viability. Further research into the complexities of the cytoskeleton promises to yield valuable insights into disease mechanisms and potential therapeutic targets.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Polymerization
The process by which small molecules (monomers) combine to form a larger molecule (polymer). This is a key process in the assembly of cytoskeletal filaments.
Dynamic Instability
A characteristic of microtubules where they rapidly switch between phases of growth and shrinkage, driven by the GTP hydrolysis of tubulin.

Key Statistics

Approximately 20-30% of the total cellular protein mass is dedicated to the cytoskeleton (Alberts et al., Molecular Biology of the Cell, 6th edition, 2015).

Source: Alberts et al., Molecular Biology of the Cell, 6th edition (2015)

Mutations in genes encoding cytoskeletal proteins are implicated in over 50% of human cancers (according to the National Cancer Institute, knowledge cutoff 2023).

Source: National Cancer Institute (2023)

Examples

Neutrophil Extracellular Traps (NETs)

Neutrophils, a type of white blood cell, utilize actin polymerization to form NETs – web-like structures released to trap and kill pathogens. This demonstrates the cytoskeleton’s role in immune defense.

Frequently Asked Questions

What are motor proteins and how do they interact with the cytoskeleton?

Motor proteins (e.g., kinesin, dynein, myosin) use ATP hydrolysis to move along cytoskeletal filaments, transporting cargo or generating force. Kinesin and dynein move along microtubules, while myosin moves along actin filaments.

Topics Covered

BiologyCell BiologyCell StructureCellular ProcessesMolecular Biology