UPSC MainsBOTANY-PAPER-II202310 Marks150 Words
Q3.

Characteristics of triplet codon

How to Approach

This question requires a detailed understanding of the genetic code and the characteristics of triplet codons. The answer should focus on the properties that make codons functional units of genetic information. Key areas to cover include the universality, degeneracy, non-overlapping, comma-free, and ambiguous nature of codons. A structured approach, defining codons and then elaborating on each characteristic with examples, will be effective. The answer should be concise, adhering to the word limit, and demonstrate a clear grasp of fundamental genetics principles.

Model Answer

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Introduction

The genetic code is the set of rules by which information encoded within genetic material (DNA or RNA sequences) is translated into proteins by living cells. This translation relies on codons, which are sequences of three nucleotides that specify a particular amino acid or a stop signal during protein synthesis. Understanding the characteristics of these triplet codons is fundamental to comprehending the mechanisms of gene expression and the flow of genetic information. These characteristics ensure the accurate and efficient translation of genetic information into functional proteins.

Characteristics of Triplet Codons

Codons are three-nucleotide sequences that specify a particular amino acid during protein synthesis. Several key characteristics define their function:

1. Universality (Near-Universal)

The genetic code is almost universal, meaning that the same codons specify the same amino acids in nearly all organisms, from bacteria to humans. This suggests a common evolutionary origin of life. However, minor variations exist in mitochondrial DNA and some protozoans.

2. Degeneracy (Redundancy)

Most amino acids are specified by more than one codon. This is known as degeneracy or redundancy. For example, leucine is coded by UUA, UUG, CUU, CUC, CUA, and CUG. This redundancy provides some protection against the harmful effects of mutations.

3. Non-Overlapping

Codons are read sequentially and do not overlap. Each nucleotide is part of only one codon. For instance, in the sequence AUGUUC, AUG and UUC are read as separate codons, not AUG and GUU.

4. Comma-Free (No Punctuation)

The genetic code is comma-free, meaning there are no intervening nucleotides between codons. The code is read continuously from one end of the mRNA to the other, without any gaps or punctuation marks.

5. Ambiguity (Wobble Hypothesis)

The wobble hypothesis, proposed by Francis Crick, explains some of the degeneracy. It suggests that the third nucleotide in a codon can sometimes pair with more than one base in the anticodon of tRNA. This allows a single tRNA molecule to recognize multiple codons that differ only in their third base. For example, the codon GCU and GCC both code for Alanine and can be read by the same tRNA.

6. Directionality

The genetic code is read in a 5’ to 3’ direction, corresponding to the direction of mRNA synthesis and translation. This directionality is crucial for the correct interpretation of the codon sequence.

Table summarizing codon characteristics:

Characteristic Description
Universality Almost the same in all organisms
Degeneracy Multiple codons for most amino acids
Non-Overlapping Codons are read sequentially without overlap
Comma-Free No gaps between codons
Ambiguity Wobble base pairing allows some flexibility

Conclusion

In conclusion, the characteristics of triplet codons – universality, degeneracy, non-overlapping nature, comma-free structure, and ambiguity – are essential for the accurate and efficient translation of genetic information. These features ensure that the genetic code functions reliably across diverse organisms, enabling the synthesis of proteins necessary for life. Understanding these properties is crucial for comprehending the complexities of molecular biology and genetic processes.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Codon
A sequence of three nucleotides in mRNA that specifies a particular amino acid or a stop signal during protein synthesis.
Wobble Hypothesis
Proposed by Francis Crick, this hypothesis explains why some tRNAs can recognize multiple codons differing in the third base, due to flexible base pairing rules.

Key Statistics

There are 64 possible codons (4<sup>3</sup>), with 61 coding for amino acids and 3 serving as stop signals.

Source: Molecular Biology of the Gene (Watson et al., 2014 - knowledge cutoff)

Approximately 99.9% of the genetic code is conserved across all life forms.

Source: Nature Reviews Genetics (2010 - knowledge cutoff)

Examples

Phenylalanine

The amino acid phenylalanine is specified by two codons: UUU and UUC, demonstrating the degeneracy of the genetic code.

Frequently Asked Questions

What is the significance of the start codon?

The start codon, AUG, signals the beginning of translation and also codes for the amino acid methionine. It is crucial for initiating protein synthesis.

Topics Covered

BiologyGeneticsGenetic CodeProtein SynthesisMolecular Biology