UPSC MainsMEDICAL-SCIENCE-PAPER-I202410 Marks
Q8.

Discuss the sources and Recommended Daily Allowance (RDA) of vitamin B12. Briefly discuss the absorption of vitamin B12 in the GIT and the clinical manifestations of the disorder of absorption of vitamin B12.

How to Approach

This question requires a detailed understanding of vitamin B12 metabolism. The approach should be structured as follows: Begin by defining vitamin B12 and its importance. Then, discuss its dietary sources and the RDA. Next, elaborate on the complex process of B12 absorption in the gastrointestinal tract, highlighting the roles of intrinsic factor and various transporters. Finally, detail the clinical manifestations arising from impaired B12 absorption, linking them to the underlying biochemical defects. A clear and concise explanation of Pernicious anemia is crucial.

Model Answer

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Introduction

Vitamin B12, also known as cobalamin, is a water-soluble vitamin essential for numerous metabolic processes, including DNA synthesis, red blood cell formation, and neurological function. Deficiency can lead to severe and irreversible neurological damage and megaloblastic anemia. Obtaining sufficient B12 through diet alone can be challenging, as it’s primarily found in animal products. Understanding its sources, recommended intake, absorption mechanisms, and the consequences of malabsorption is crucial for effective clinical management. This answer will detail these aspects, focusing on the physiological processes and clinical implications of B12 deficiency.

Sources of Vitamin B12

Vitamin B12 is synthesized by microorganisms and is not found in plants, except when fortified. The primary dietary sources include:

  • Animal Products: Liver, meat (especially beef and pork), poultry, fish (salmon, tuna), eggs, and dairy products.
  • Fortified Foods: Breakfast cereals, nutritional yeast, and plant-based milk alternatives are often fortified with B12.
  • Supplements: Available in various forms, including cyanocobalamin, methylcobalamin, and hydroxocobalamin.

Recommended Daily Allowance (RDA) of Vitamin B12

The RDA for vitamin B12 varies with age and physiological status:

Age Group RDA (µg/day)
Infants (0-6 months) 0.4
Infants (7-12 months) 0.5
Children (1-3 years) 0.9
Children (4-8 years) 1.2
Children (9-13 years) 1.8
Adolescents & Adults (14+ years) 2.4
Pregnant Women 2.6
Lactating Women 2.8

Individuals with conditions affecting B12 absorption (e.g., pernicious anemia, Crohn’s disease) may require higher doses, often administered via injection or high-dose oral supplements.

Absorption of Vitamin B12 in the Gastrointestinal Tract

Vitamin B12 absorption is a complex, multi-step process:

  1. Release from Food: In the stomach, gastric acid and pepsin release B12 from proteins.
  2. Binding to R-protein: B12 binds to R-protein (haptocorrin), secreted by salivary glands and gastric parietal cells. This protects B12 from degradation in the acidic environment.
  3. Pancreatic Protease Action: In the duodenum, pancreatic proteases digest R-protein, releasing B12.
  4. Binding to Intrinsic Factor (IF): B12 then binds to intrinsic factor, a glycoprotein secreted by gastric parietal cells. This is a crucial step.
  5. Absorption in the Ileum: The B12-IF complex travels to the ileum, where it binds to specific receptors (cubilin) on the enterocytes.
  6. Transport into Cells: The complex is internalized via receptor-mediated endocytosis.
  7. Release and Transport to Blood: Inside the enterocytes, B12 is released from IF and binds to transcobalamin II (TCII), which transports it to the bone marrow and other tissues.

Clinical Manifestations of Vitamin B12 Absorption Disorders

Disorders affecting B12 absorption lead to deficiency, resulting in a range of clinical manifestations:

  • Megaloblastic Anemia: Characterized by large, immature red blood cells due to impaired DNA synthesis. Symptoms include fatigue, weakness, and shortness of breath.
  • Neurological Symptoms: These are often the most serious and potentially irreversible consequences of B12 deficiency. They include:
    • Peripheral neuropathy (numbness, tingling in hands and feet)
    • Subacute combined degeneration of the spinal cord (difficulty walking, loss of balance)
    • Cognitive impairment (memory loss, confusion)
    • Psychiatric symptoms (depression, irritability)
  • Glossitis: A sore, red tongue.
  • Gastrointestinal Symptoms: Loss of appetite, nausea, and diarrhea.

Pernicious Anemia: An autoimmune disorder where the body attacks parietal cells in the stomach, leading to a lack of intrinsic factor. This results in impaired B12 absorption and is a common cause of B12 deficiency. Diagnosis often involves detecting anti-parietal cell antibodies and anti-intrinsic factor antibodies.

Conclusion

Vitamin B12 is vital for maintaining overall health, and its absorption is a complex process dependent on multiple factors. Deficiency, often stemming from impaired absorption, can lead to severe and debilitating consequences, particularly neurological damage. Understanding the sources, RDA, absorption mechanisms, and clinical manifestations of B12 deficiency is crucial for timely diagnosis and effective treatment, often involving lifelong B12 supplementation. Early intervention is key to preventing irreversible neurological complications.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Intrinsic Factor
A glycoprotein produced by parietal cells in the stomach, essential for the absorption of vitamin B12 in the ileum. It binds to B12, forming a complex that can be absorbed by intestinal cells.
Transcobalamin II (TCII)
A transport protein responsible for delivering vitamin B12 to tissues throughout the body, particularly the bone marrow for red blood cell production.

Key Statistics

Approximately 1.5-15% of adults over 50 years of age have vitamin B12 deficiency due to decreased gastric acid production and reduced intrinsic factor secretion.

Source: National Institutes of Health (NIH), Office of Dietary Supplements (as of knowledge cutoff 2023)

Approximately 20-40% of individuals with Crohn’s disease experience vitamin B12 malabsorption due to damage to the ileum, the primary site of B12 absorption.

Source: Crohn's & Colitis Foundation (as of knowledge cutoff 2023)

Examples

Vegan Diet and B12 Deficiency

Strict vegans are at high risk of B12 deficiency as plant-based foods do not naturally contain significant amounts of the vitamin. They must rely on fortified foods or supplements to meet their daily requirements.

Frequently Asked Questions

Can vitamin B12 deficiency be reversed?

Neurological damage from long-term B12 deficiency may be irreversible, but early diagnosis and treatment with B12 supplementation can often improve symptoms and prevent further progression. Anemia typically responds well to treatment.

Topics Covered

BiochemistryPhysiologyVitamin MetabolismAbsorptionDeficiency Diseases