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Q9.

What is sex-determination? Give an account of genic balance theory of sex-determination in Drosophila with examples from abnormal karyotypes.

How to Approach

This question requires a detailed understanding of sex determination mechanisms, specifically focusing on the genic balance theory proposed by Bridges. The answer should begin with a general definition of sex determination, then delve into the specifics of the genic balance theory, explaining the role of X:A ratio. Crucially, the answer must illustrate the theory with examples of abnormal karyotypes (e.g., XXY, X0, etc.) and their resulting phenotypes in *Drosophila*. A clear, step-by-step explanation with illustrative examples is key to scoring well.

Model Answer

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Introduction

Sex determination, the biological process that determines the sex of an individual, is a fundamental aspect of reproduction. While various mechanisms exist across species – including chromosomal, hormonal, and environmental factors – the genic balance theory provides a unique explanation for sex determination in *Drosophila melanogaster*. Proposed by Calvin Bridges in the 1920s, this theory posits that sex is not determined by the presence or absence of a specific chromosome (like the Y chromosome in humans), but rather by the ratio of X chromosomes to autosomes (non-sex chromosomes). This ratio dictates the developmental pathway towards either male or female characteristics.

Sex Determination: An Overview

Sex determination is the process by which the sex of an organism is established. Several mechanisms are known, including:

  • Chromosomal Determination (e.g., Humans): Presence of Y chromosome determines maleness.
  • Hormonal Determination (e.g., some reptiles): Temperature during incubation influences sex.
  • Genic Balance Theory (e.g., Drosophila): Ratio of X chromosomes to autosomes determines sex.

The Genic Balance Theory in Drosophila

Bridges proposed that sex in Drosophila is determined by the balance between ‘female-determining’ genes located on the X chromosome and ‘male-determining’ genes distributed on the autosomes. He termed the effective genetic contribution of the X chromosome as ‘X’ and the genetic contribution of all autosomes as ‘A’. The sex of an individual is determined by the ratio X/A.

Key Principles:

  • A ratio of X/A = 1.0 results in a female.
  • A ratio of X/A = 0.5 results in a male.
  • Any deviation from these ratios leads to intersex phenotypes.

The ‘female’ genes on the X chromosome produce a female-determining factor, while the ‘male’ genes on the autosomes produce a male-determining factor. The balance between these factors determines the sexual phenotype.

Normal Karyotypes and Sex Determination

A normal Drosophila female has two X chromosomes (XX) and a normal set of autosomes. Therefore, X/A = 2/2 = 1.0, resulting in a female. A normal male has one X chromosome (X) and a normal set of autosomes. Therefore, X/A = 1/2 = 0.5, resulting in a male.

Abnormal Karyotypes and Phenotypes

The power of the genic balance theory lies in its ability to explain the phenotypes observed in individuals with abnormal chromosome numbers. Let's examine some examples:

Karyotype X/A Ratio Phenotype Explanation
XXY 3/3 = 1.0 Female The increased X/A ratio favors female development, overriding the presence of the Y chromosome.
X0 1/2 = 0.5 Male Despite the absence of a second X chromosome, the X/A ratio remains at 0.5, resulting in a male.
XXX 3/2 = 1.5 Metafemale (sterile female) The significantly increased X/A ratio leads to a sterile female with broader abdomen and other female characteristics.
XYY 1/3 = 0.33 Metamale (sterile male) The decreased X/A ratio leads to a sterile male with smaller size and other male characteristics.
XX 2/2 = 1.0 Female Normal female.

These examples demonstrate that the absolute number of X or Y chromosomes is less important than the relative ratio of X chromosomes to autosomes. The theory accurately predicts the sex phenotype even in cases where the typical chromosomal sex determinants are altered.

Conclusion

The genic balance theory of sex determination in *Drosophila* provides a compelling explanation for how sex is determined not by the presence of specific chromosomes, but by the quantitative balance of genes on the X chromosomes relative to the autosomes. The theory’s predictive power, as demonstrated by its ability to explain phenotypes resulting from abnormal karyotypes, solidified its importance in the field of genetics. While more complex mechanisms are now known to influence sex determination in other organisms, the genic balance theory remains a cornerstone of our understanding of sex determination and a classic example of how genetic ratios can dictate developmental pathways.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Autosomes
Chromosomes that are not sex chromosomes; they contain genes for general body characteristics.
Karyotype
The number and appearance of chromosomes in the nucleus of an eukaryotic cell.

Key Statistics

Approximately 80% of insects exhibit sex determination mechanisms that are not based on the presence or absence of a Y chromosome, highlighting the diversity of sex determination systems.

Source: Whittaker, M. M. (2017). Sex determination in insects. Current Biology, 27(17), R954–R959.

Studies have shown that the X chromosome in *Drosophila* contains approximately 15% of the total genes, but contributes significantly to sex determination.

Source: Ashburner, M. (1989). Drosophila genetics: a collection of papers on the genetic and developmental biology of Drosophila melanogaster. Oxford University Press.

Examples

Honeybee Sex Determination

Honeybees exhibit a haplodiploid sex-determination system where females develop from fertilized eggs (diploid) and males develop from unfertilized eggs (haploid), demonstrating a different mechanism than the genic balance theory.

Frequently Asked Questions

Does the genic balance theory apply to humans?

No, the genic balance theory is specific to *Drosophila*. Human sex determination is primarily based on the presence or absence of the Y chromosome, specifically the SRY gene located on the Y chromosome.

Topics Covered

BiologyGeneticsSex ChromosomesInheritanceDevelopmental Biology