UPSC MainsBOTANY-PAPER-II201212 Marks
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Q21.

Distinguish between passive and facilitated diffusion.

How to Approach

This question requires a comparative analysis of two fundamental types of membrane transport – passive and facilitated diffusion. The answer should clearly define both processes, highlight their similarities and differences, focusing on the role of membrane proteins, energy requirement, and the types of molecules transported. A tabular comparison will be highly effective. The answer should demonstrate a strong understanding of cell biology principles.

Model Answer

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Introduction

Cellular transport is crucial for maintaining homeostasis and carrying out essential life processes. Membrane transport mechanisms allow substances to move across the cell membrane, which is selectively permeable. Two important mechanisms are passive diffusion and facilitated diffusion, both of which are types of passive transport, meaning they do not require the cell to expend metabolic energy. However, they differ significantly in their mechanisms and the types of molecules they transport. Understanding these differences is fundamental to comprehending cellular function and physiological processes.

Passive Diffusion

Passive diffusion, also known as simple diffusion, is the movement of substances across a cell membrane down their concentration gradient – from an area of high concentration to an area of low concentration. This process doesn’t require the assistance of membrane proteins. The rate of diffusion is influenced by factors like concentration gradient, temperature, pressure, and the permeability of the membrane to the substance.

  • Mechanism: Movement directly through the phospholipid bilayer.
  • Energy Requirement: No energy expenditure (ATP) required.
  • Membrane Proteins: Not involved.
  • Specificity: Non-specific; any molecule that can dissolve in the lipid bilayer can diffuse.
  • Examples: Oxygen (O2), Carbon Dioxide (CO2), and small nonpolar molecules like ethanol.

Facilitated Diffusion

Facilitated diffusion is also the movement of substances down their concentration gradient, but it requires the assistance of specific membrane proteins to cross the cell membrane. These proteins can be either channel proteins or carrier proteins.

  • Mechanism: Movement through membrane proteins (channel or carrier proteins).
  • Energy Requirement: No energy expenditure (ATP) required.
  • Membrane Proteins: Essential; either channel or carrier proteins.
  • Specificity: Highly specific; proteins bind only to certain molecules.
  • Examples: Glucose, amino acids, and ions (Na+, K+, Cl-).

Comparison Table

Feature Passive Diffusion Facilitated Diffusion
Concentration Gradient Movement down the gradient Movement down the gradient
Energy Requirement None None
Membrane Proteins Not Required Required (Channel or Carrier)
Specificity Non-specific Highly Specific
Rate of Transport Slower Faster (due to protein assistance)
Saturation Does not exhibit saturation Can exhibit saturation (limited number of proteins)
Examples O2, CO2, Ethanol Glucose, Amino Acids, Ions

Channel Proteins vs. Carrier Proteins

Within facilitated diffusion, channel proteins form a pore through the membrane, allowing specific ions or molecules to pass through. Carrier proteins bind to the molecule and undergo a conformational change to transport it across the membrane. Channel proteins are generally faster, while carrier proteins exhibit higher specificity and can be subject to saturation.

The rate of facilitated diffusion is limited by the number of available carrier or channel proteins. Once all protein binding sites are occupied, the transport rate reaches a maximum, a phenomenon known as saturation. Passive diffusion does not exhibit saturation as the rate is directly proportional to the concentration gradient.

Conclusion

In summary, both passive and facilitated diffusion are vital processes for cellular transport, relying on the concentration gradient and not requiring energy expenditure. However, facilitated diffusion utilizes membrane proteins for specificity and increased transport rates, while passive diffusion relies on the inherent permeability of the lipid bilayer. Understanding these distinctions is crucial for comprehending how cells regulate their internal environment and carry out essential functions. Further research into the regulation of these transport proteins is ongoing, potentially leading to novel therapeutic interventions for various diseases.

Answer Length

This is a comprehensive model answer for learning purposes and may exceed the word limit. In the exam, always adhere to the prescribed word count.

Additional Resources

Key Definitions

Osmosis
A specific type of diffusion involving the movement of water across a semi-permeable membrane from a region of higher water potential to a region of lower water potential.
Membrane Permeability
The extent to which a membrane allows substances to pass through it. It is influenced by the chemical properties of the membrane and the substance, as well as temperature and pressure.

Key Statistics

The human body is approximately 55-78% water, highlighting the importance of water transport across cell membranes (Source: H.H. Mitchell, Journal of Biological Chemistry, 1948).

Source: H.H. Mitchell, Journal of Biological Chemistry, 1948

Approximately 99% of the molecules crossing the cell membrane do so through transport proteins (Source: Alberts et al., Molecular Biology of the Cell, 6th edition, 2015).

Source: Alberts et al., Molecular Biology of the Cell, 6th edition, 2015

Examples

Glucose Transport in Red Blood Cells

Glucose enters red blood cells via facilitated diffusion using GLUT1 transporter proteins. This is essential for providing energy to these cells, which lack mitochondria and rely on glycolysis for ATP production.

Frequently Asked Questions

What happens if a molecule tries to diffuse against its concentration gradient?

Moving a molecule against its concentration gradient requires energy and is therefore not diffusion. This process is called active transport and utilizes membrane proteins and ATP.

Topics Covered

BiologyCell BiologyMembrane TransportCellular ProcessesDiffusion